Articles
PRODUCTION OF ROOT BIOMASS AND SECONDARY METABOLITES THROUGH ADVENTITIOUS ROOT CULTURES OF ECHINACEA PURPUREA IN BIOREACTORS
Article number
829_9
Pages
73 – 78
Language
English
Abstract
Adventitious roots of Echinacea purpurea, an important medicinal plant, were cultured in balloon type airlift bioreactors (5 L capacity). Investigations were made to optimize inoculum density and aeration rate affecting the accumulation of biomass and secondary metabolites.
The greatest increment of biomass as well as metabolite content occurred at the inoculum density of 7 g/L fresh weight (FW) and the aeration volume of 0.1 vvm.
Under culture conditions 60.7 mg/g dry weight (DW) of phenolics and 38.8 mg/g DW of flavonoids were produced in the adventitious roots after five weeks of culture.
Cichoric acid content was highest (26.64 mg/g DW) among the caffeic acid derivatives.
The results suggest that the accumulation of biomass and secondary metabolites were maximized by optimizing inoculum density and aeration volume in bioreactor cultures of Echinacea purpurea.
The greatest increment of biomass as well as metabolite content occurred at the inoculum density of 7 g/L fresh weight (FW) and the aeration volume of 0.1 vvm.
Under culture conditions 60.7 mg/g dry weight (DW) of phenolics and 38.8 mg/g DW of flavonoids were produced in the adventitious roots after five weeks of culture.
Cichoric acid content was highest (26.64 mg/g DW) among the caffeic acid derivatives.
The results suggest that the accumulation of biomass and secondary metabolites were maximized by optimizing inoculum density and aeration volume in bioreactor cultures of Echinacea purpurea.
Authors
E.J. Hahn, C.H. Wu, K.Y. Paek
Keywords
adventitious root, caffeic acid derivatives, Echinacea purpurea
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