Home > VI International Symposium on In Vitro Culture and Horticultural Breeding > IN VITRO FLOWERING AND PROPAGATION OF BULBOPHYLLUM AURICOMUM LINDL., THE ROYAL FLOWER OF MYANMAR

Articles

IN VITRO FLOWERING AND PROPAGATION OF BULBOPHYLLUM AURICOMUM LINDL., THE ROYAL FLOWER OF MYANMAR

Article number

829_14

Pages

105 – 111

Language

English

Abstract

Reproducible protocols for micropropagation of B. auricomum Lindl. were developed in the present study.
Seeds from three-month-old immature seed capsules were aseptically cultured on Murashige and Skoogs (MS) and modified Knudson C (KC) medium.
MS medium was found to be the most effective culture medium for seed germination, and a high rate of protocorms formation, multiplication, and differentiation into seedlings.
The combination of 1.0 mg/L 6-Benzylaminopurine (BAP) with 0.5 mg/L α-Naphthaleneacetic acid (NAA) induced maximum number of multiple shoots (15.0±1.7) and optimal rooting (3.0±0.7) of plantlets was obtained in 1.0 mg/L kinetin (KIN) and NAA 2.0 mg/L. In vitro flowering was observed within six months after seed sowing, with the highest percentage (50±15.8%) of flowering observed in MS medium with 1.0 mg/L BAP + 0.5 mg/L NAA; with 2.0 mg/L BAP + 1.0 mg/L NAA or with 0.5 mg/L BAP + 2.0 mg/L NAA. In vitro-developed flowers from different treatments were dissected and compared with flowers of the field grown plants.

Publication

VI International Symposium on In Vitro Culture and Horticultural Breeding

Authors

M.M.M. Than, A. Pal, S. Jha

Keywords

Full text

https://doi.org/10.17660/ActaHortic.2009.829.14

Online Articles (69)

1. RECENT ADVANCES IN THE APPLICATION OF IN VITRO SYSTEMS TO PLANT IMPROVEMENT

J.M. Dunwell

2. MODIFICATION OF HORMONE SIGNALING FOR IMPROVEMENT OF PLANTS

M.R. Godge | D. Kumar | Hyun Sook Pai | P.P. Kumar

3. SOMATIC EMBRYOGENESIS IN BEGONIA X HIEMALIS FOTSCH. IN VITRO

A. Awal | R.M. Taha | N.A. Hasbullah

4. SECONDARY SOMATIC EMBRYOGENESIS IN BANANA CIEN-BTA-03 (MUSA SP. AAAA) AND REGENERATION OF PLANTS

M. Ramírez-Villalobos | E. de García

5. DEVELOPMENT OF MICROCOLONIES IN PROTOPLAST CULTURE OF SPATHIPHYLLUM WALLISII

T. Eeckhaut | B. Duquenne | P. Laksmanan | J. Van Huylenbroeck

6. IN VITRO ASSISTED BREEDING OF EVERGREEN AZALEA AS A TOOL FOR ASSORTMENT EXTENSION

T. Eeckhaut | E. De Keyser | J. Van Huylenbroeck

7. IN VITRO ANTHER AND ISOLATED MICROSPORE CULTURE AS TOOLS IN SWEET AND SPICE PEPPER BREEDING

A. Gémes Juhász | Z. Kristóf | P. Vági | C. Lantos | J. Pauk

8. IN VITRO BREEDING PROVIDES NEW AND UNIQUE OPPORTUNITIES FOR CONVENTIONAL BREEDING

J.W. Grosser | F.G. Gmitter Jr.

9. PRODUCTION OF ROOT BIOMASS AND SECONDARY METABOLITES THROUGH ADVENTITIOUS ROOT CULTURES OF ECHINACEA PURPUREA IN BIOREACTORS

E.J. Hahn | C.H. Wu | K.Y. Paek

10. VERMICULITE IMPROVES EARLY DEVELOPMENT AND SURVIVAL OF LOW CHILL STONE-FRUIT EMBRYOS RESCUED IN VITRO

S. Hamill | S. Promchot | G. Bignell | J. Giles | B. Topp

11. EVALUATING MUTATION BREEDING METHODS TO FAST-TRACK THE DOMESTICATION OF TWO AUSTRALIAN NATIVE CALANDRINIA SPECIES FOR ORNAMENTAL HORTICULTURE

D.K. Harrison | P. Wickramasinghe | M.E. Johnston | D.C. Joyce

12. PRODUCTION OF SYNTHETIC SEEDS FROM MICRO SHOOTS AND SOMATIC EMBRYOS OF GERBERA JAMESONII BOLUS EX. HOOK F.

R.M. Taha | N.A. Hasbullah | A. Awal

13. IN VITRO CULTURE OF PERESKIA BLEO

N. Jaafar Sidik | M.S. Norihan | K. Shafii

14. IN VITRO FLOWERING AND PROPAGATION OF BULBOPHYLLUM AURICOMUM LINDL., THE ROYAL FLOWER OF MYANMAR

M.M.M. Than | A. Pal | S. Jha

15. ECO-TURF: WATER USE EFFICIENT TURFGRASSES FROM AUSTRALIAN BIODIVERSITY

16. METABOLIC ENGINEERING OF THE MORPHINE PATHWAY IN OPIUM POPPY

P.J. Larkin

17. CORRELATION BETWEEN BROWNING, TOTAL PHENOLIC CONTENT, POLYPHENOL OXIDASE AND SEVERAL ANTIOXIDATION ENZYMES DURING PISTACHIO TISSUE CULTURE

Pingsheng Leng | Shuchai Su | Fang Wei | Fei Yu | Yunfeng Duan

18. CITRUS GERMPLASM ENHANCEMENT VIA CELL ENGINEERING AND GENETIC ENGINEERING – PROGRESS REPORT

Ji-Hong Liu | Xing-Zheng Fu | Xi-San Huang | Jing Wang | Qi-Jun Fan

19. SYNERGISTIC PROMOTION OF GIBBERELLIN AND CYTOKININ ON REGENERATION OF FLORAL BUDS FROM PETAL SEGMENTS IN VITRO IN SINNINGIA SPECIOSA HIERN

J.L. Pang | L.L. Wang | T.H. Xiang | J.Q. Hu | D. Zhong

20. APICAL DOMINANCE IN ALSTROEMERIA CULTURED IN VITRO

P. Pumisutapon | R.G.F Visser | G.-J. De Klerk

21. AGROBACTERIUM TUMEFACIENS-MEDIATED TRANSFORMATION OF ONCIDIUM AND ODONTOGLOSSUM WITH THE MUTATED ETHYLENE RECEPTOR ETR1-1

B. Raffeiner | T. Winkelmann | M. Serek

22. BACTERIALLY-INDUCED GROWTH PROMOTION OF MICROPROPAGATED GINGER

E. Rames | S. Hamill | I. Kurtböke

23. IN VIVO FUNCTIONAL ASSAY OF WUS ORTHOLOG IN TOBACCO

S.Z. Rashid | M. Kyo

24. EPIGENETIC EFFECTS IN INDUCED IN VITRO TETRAPLOIDS PASSION FRUIT (PASSIFLORA EDULIS SIMS.)

M.M. do Rêgo | E.R. do Rêgo | W.C. Otoni | C.H. Bruckner

25. THE OIL PALM “MANTLED” SOMACLONAL VARIATION: A MODEL FOR EPIGENETIC STUDIES IN HIGHER PLANTS

A. Rival | E. Jaligot | T. Beulé | J.W. Tregear | J. Finnegan

26. ENHANCING WAXFLOWER BREEDING EFFICIENCY THROUGH EARLY EMBRYO RESCUE

F. Shan | K. Seaton

27. TISSUE CULTURE AND SALT STRESS IN CHRYSANTHEMUM MORIFOLIUM

28. CRYOPRESERVATION BY ENCAPSULATION-DEHYDRATION OF EMBRYOGENIC CALLUS OF WILD CROCUS (CROCUS HYEMALIS AND C. MOABITICUS)

29. MORPHOLOGICAL AND MOLECULAR ANALYSIS OF INTRACULTIVAR VARIATION IN INDIAN MANGO (MANGIFERA INDICA L.) CULTIVARS

S. Singh | A.B. Gaikwad | J.L. Karihaloo

30. MICROPROPAGATION OF VEGETATIVELY PROPAGATED CROPS: ACCELERATING RELEASE OF NEW CULTIVARS AND PROVIDING AN IMPORTANT SOURCE OF CLEAN PLANTING MATERIAL

S.D. Hamill | J.A. Moisander | M.K. Smith

31. DATE PALM CROP IMPROVEMENT VIA T X D HYBRIDIZATION INTEGRATED WITH IN VITRO CULTURE TECHNIQUE

C. Sudhersan | Y. Al-Shayji | S. Jibi Manuel

32. INFLUENCE OF CARBOHYDRATE SOURCE ON THE IN VITRO FLOWERING OF STURT’S DESERT PEA (SWAINSONA FORMOSA)

T. Tapingkae | P. Kristiansen | A. Taji

33. THE IMPORTANCE OF GERMPLASM DEVELOPMENT AND EXCHANGE IN MEETING THE CHALLENGES OF THE 21^ST CENTURY IN THE PACIFIC REGION

M.B. Taylor | V.S. Tuia

34. ESTABLISHING METHODS FOR INTER-GENERIC HYBRIDIZATION OF AUSTRALIAN NATIVE CALANDRINIA AND EXOTIC PORTULACA SPECIES FOR ORNAMENTAL HORTICULTURE

P. Wickramasinghe | D.K. Harrison | M.E. Johnston

35. IN VITRO INDUCTION OF AUTOTETRAPLOID ACTINIDIA PLANTS AND THEIR FIELD EVALUATION FOR CROP IMPROVEMENT

Jin-Hu Wu | A.R. Ferguson | B.G. Murray

36. CHARACTERIZATION OF CHLOROSIS-RESISTANT APPLE MUTANTS OF TRANSGENIC FRO2

37. CAN ARABIDOPSIS AP1 GENE SHORTEN THE JUVENILITY OF APPLE?

L.H. Zhu | X.Y. Li | M. Welander

38. THE EFFECT OF NAA AND KIN ON THE INDUCTION OF CALLUS FROM RETAMA RAETAM PLANTS

I.M. Abobaker | G.E. Elnour | S.A. Mahfouz

39. VARIETAL IDENTIFICATION AND GENETIC PURITY ASSESSMENT OF F₁ HYBRID SEEDS USING RAPD MARKERS IN FABA BEAN (VICIA FABA L.)

S.S. Alghamdi

40. EVALUATION OF GENETIC DIVERSITY IN CAPSICUM SPP. AS REVEALED BY RAPD MARKERS

M. Bahrami Rad | M.E. Hassani | A. Mohammadi | SH. Lessan | S. Ghazi Zade

41. INVESTIGATIONS OF ALTERNATIVE IN VITRO ROOTING METHODS WITH RARE AND RECALCITRANT PLANTS

E. Bunn

42. TRANSFORMATION OF DENDROBIUM PRIMULINUM LINDL. WITH ANTISENSE ACC OXIDASE

S. Bunnag | K. Duangsee

43. A DECONTAMINATION PROCEDURE FOR IN VITRO CULTURE OF LILIUM LONGIFLORUM CV. ‘DOZZEL’ SCALE EXPLANTS

B. Ghoreyshi | R. Naderi | S.A. Ghaem Maghami | S. Zeyni

44. THE EFFECT OF DIFFERENT THIDIAZURON AND INDOLE BUTYRIC ACID CONCENTRATIONS ON IN VITRO PROPAGATION OF ‘GRAND NAIN’ AND ‘BASRAI’ BANANA CULTIVARS

H. Gubbuk | M. Pekmezci

45. EFFECTS OF VARIOUS SUCROSE CONCENTRATION AND ANCYMIDOL ON SHOOT ELONGATION AND WATER STATUS OF ASPARAGUS IN VITRO

R. Honjo | T. Ikeda | A. Taji | A. Uragami

46. THE EFFECT OF VARIOUS HORMONE TYPES ON IN VITRO PROPAGATION OF STRAWBERRY

N. Adak | L. Kaynak | M. Pekmezci | H. Gubbuk

47. INVESTIGATING THE IN VITRO GROWTH INHIBITION OF ORYZALIN-TREATED GERBERA JAMESONII

Z. Ghayoor Karimiani | A. Bagheri | G.H. Davarynejad | M. Jafarkhani Kermani

48. A REFINED TISSUE CULTURE MEDIUM FOR IN VITRO PROLIFERATION OF APPLE ROOTSTOCKS

M. Jafarkhani Kermani | Z.S. Hosseini | A.A. Habashi

49. ESTABLISHMENT OF EMBRYOGENIC CELL SUSPENSION AND FIELD EVALUATION OF REGENERANTS OF ZINGIBER ZERUMBET SMITH

F.D. Idris | M. Jalil | H. Ibrahim | N. Khalid

50. REGENERATION SYSTEM AND AGROBACTERIUM-MEDIATED TRANSFORMATION OF SWEET CLOVER WITH HERBICIDE-RESISTANCE GENE

51. SOMATIC EMBRYOGENESIS AND PRODUCTION OF ARTIFICIAL SEEDS IN SAINTPAULIA IONANTHA WENDL.

R.M. Taha | N. Daud | N.A. Hasbullah | A. Awal

52. IN VITRO MUTAGENESIS FOR SALT TOLERANT RAPESEED (BRASSICA NAPUS L.) USING GAMMA IRRADIATION

E. Mosleh | A. Mohammadi | M. Omidi | S.J. Rastegari

53. MICROBIAL CONTAMINATION IN EXPLANTS OF BANANA CULTIVARS ‘GALIL 18’ AND ‘TROPICAL’

54. PRELIMINARY REPORT ON AGROBACTERIUM-MEDIATED GENETIC TRANSFORMATION OF BEGONIA REX AND SAINTPAULIA SPP.

S. Ohki | Y. Hashimoto | M. Ohno

55. IN VITRO RESPONSE OF DIFFERENT EXPLANTS’ TYPES ON SHOOT AND ROOT DEVELOPMENT OF GINGER

H. Rajani | S.S. Patil

56. SLOW GROWTH IN VITRO CONSERVATION OF DENDROBIUM DRACONIS RCHB. F.: EFFECT OF BLACK KWAO KRUA (MUCUNA COLLETTII LACE)

N. Rangsayatorn | K. Jina | S. Pampasit

57. PEG FUSION OF CHAMELAUCIUM UNCINATUM PROTOPLASTS

K. Ratanasanobon | K.A. Seaton

58. IN VITRO MICROGRAFTING PROTOCOL IN LYCOPERSICON ESCULENTUM

M.M. do Rêgo | E.R. do Rêgo | O. de Lima Coutinho

59. SELECTION FOR LEAF AND PLANT SIZE AND LONGEVITY OF ORNAMENTAL PEPPERS (CAPSICUM SPP.) GROWN UNDER GREENHOUSE CONDITION

60. VARIATION IN PLOIDY AND MORPHOLOGY AMONG ANTHER-DERIVED PAPAYA PLANTS

F.K. Rimberia | S. Adaniya | Y. Ishimine | T. Etoh

61. COMMERCIAL MASS CLONAL PROPAGATION OF GRAPEVINE CV. ‘BIDANEH SEFID’: IN VITRO FRAGMENTED SHOOT APEX CULTURE OF AN ELITE GERMPLASM

S.A. Salami | A. Ebadi | Z. Zamani

62. DISTRIBUTION OF GFLV IN IRANIAN VINEYARDS AND IMPROVEMENTS TO SANITATION PROTOCOLS FOR PRODUCTION OF VIRUS-FREE GRAPEVINES

S.A. Salami | A. Ebadi | Z. Zamani | M. Koohi Habibi

63. EFFECTS OF ELECTRIC CURRENT VALUE AND TIME TREATMENTS ON ELIMINATION OF CARNATION MOTTLE VIRUS AND IN VITRO PLANTLET REGENERATION OF CARNATION (DIANTHUS CARYOPHYLLUS)

S. Sepahpour | A. Moieni | M. Shamsbakhsh | A. Baghizadeh

64. EFFECT OF CHITOSAN AND JASMONIC ACID RELATED COMPOUNDS ON SHOOT FORMATION FROM RHIZOME CULTURES OF AN ORIENTAL CYMBIDIUM SPECIES

65. UNDERSTANDING THE MECHANISMS OF GRAFT UNION FORMATION IN SOLANACEAE PLANTS USING IN VITRO TECHNIQUES

M. Kawaguchi | D. Backhouse | T. Tapingkae | A. Taji

66. NEW SHIPPING SYSTEM FOR CURCUMA HYBRID PLANTLETS CULTURED FROM TWIN-FLASKS TEMPORARY IMMERSION BIOREACTOR

N. Topoonyanont | M. Unjai | S. Jaikanta | T. Taychasinpitak

67. INTERGENERIC HYBRIDIZATION, EMBRYO RESCUE AND MOLECULAR DETECTION FOR INTERGENERIC HYBRIDS BETWEEN ASCOCENDA AND PHALAENOPSIS

C.C. Tsai | Y.C. Chiang | S.C. Huang | W.L. Liu | C.H. Chou

68. GROWTH AND WATER STATUS OF TISSUE-CULTURED STRAWBERRY PLANTS AS AFFECTED BY SALT STRESS AND DIFFERENT SUGAR CONCENTRATION IN MEDIA

Y. Okawai | T. Ikeda | A. Taji

69. ESTABLISHMENT OF IN VITRO SALVIA CELL BIOMASS FOR THE CONTROLLED PRODUCTION OF ANTIOXIDANT METABOLITES