IN VITRO FLOWERING AND PROPAGATION OF BULBOPHYLLUM AURICOMUM LINDL., THE ROYAL FLOWER OF MYANMAR

Reproducible protocols for micropropagation of B. auricomum Lindl. were developed in the present study.
Seeds from three-month-old immature seed capsules were aseptically cultured on Murashige and Skoog’s (MS) and modified Knudson C (KC) medium.
MS medium was found to be the most effective culture medium for seed germination, and a high rate of protocorms formation, multiplication, and differentiation into seedlings.
The combination of 1.0 mg/L 6-Benzylaminopurine (BAP) with 0.5 mg/L α-Naphthaleneacetic acid (NAA) induced maximum number of multiple shoots (15.0±1.7) and optimal rooting (3.0±0.7) of plantlets was obtained in 1.0 mg/L kinetin (KIN) and NAA 2.0 mg/L. In vitro flowering was observed within six months after seed sowing, with the highest percentage (50±15.8%) of flowering observed in MS medium with 1.0 mg/L BAP + 0.5 mg/L NAA; with 2.0 mg/L BAP + 1.0 mg/L NAA or with 0.5 mg/L BAP + 2.0 mg/L NAA. In vitro-developed flowers from different treatments were dissected and compared with flowers of the field grown plants.

VI International Symposium on In Vitro Culture and Horticultural Breeding

M.M.M. Than, A. Pal, S. Jha

https://doi.org/10.17660/ActaHortic.2009.829.14