Home > VI International Symposium on In Vitro Culture and Horticultural Breeding > EFFECTS OF ELECTRIC CURRENT VALUE AND TIME TREATMENTS ON ELIMINATION OF CARNATION MOTTLE VIRUS AND IN VITRO PLANTLET REGENERATION OF CARNATION (DIANTHUS CARYOPHYLLUS)

Articles

EFFECTS OF ELECTRIC CURRENT VALUE AND TIME TREATMENTS ON ELIMINATION OF CARNATION MOTTLE VIRUS AND IN VITRO PLANTLET REGENERATION OF CARNATION (DIANTHUS CARYOPHYLLUS)

Article number

829_63

Pages

395 – 398

Language

English

Abstract

The effects of current value and time treatments on plantlet regeneration were studied in this research.
Carnation stems containing at least five nodes were exposed to 0, 10, 15, or 20 milliampers (mA) for 10 or 15 minutes, then the sterilized nodal explants were cultured on MS medium.
Six traits, including stem thickness, shoot length, leaf number per shoot, shoot number per explant after two months, percentage of plantlet regeneration after two weeks, and percentage of virus-free plantlets after transferring rooted plantlets to soil, were measured.
The best result for plantlet regeneration trait was obtained in 0 and 10 miliampers respectively.
In the highest current value treatments (20 mA for 10 or 15 minutes) plantlet regeneration rates were 86.6 and 60.6%, respectively, and the highest virus-free plantlets was obtained 13.6 and 16% in Treatments of 20 mA for 10 minutes and 10 mA for 15 minutes respectively.

Publication

VI International Symposium on In Vitro Culture and Horticultural Breeding

Authors

S. Sepahpour, A. Moieni, M. Shamsbakhsh, A. Baghizadeh

Keywords

carnation, electrotherapy, in vitro culture, plant regeneration, virus disease

Full text

https://doi.org/10.17660/ActaHortic.2009.829.63

Online Articles (69)

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2. MODIFICATION OF HORMONE SIGNALING FOR IMPROVEMENT OF PLANTS

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5. DEVELOPMENT OF MICROCOLONIES IN PROTOPLAST CULTURE OF SPATHIPHYLLUM WALLISII

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8. IN VITRO BREEDING PROVIDES NEW AND UNIQUE OPPORTUNITIES FOR CONVENTIONAL BREEDING

J.W. Grosser | F.G. Gmitter Jr.

9. PRODUCTION OF ROOT BIOMASS AND SECONDARY METABOLITES THROUGH ADVENTITIOUS ROOT CULTURES OF ECHINACEA PURPUREA IN BIOREACTORS

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10. VERMICULITE IMPROVES EARLY DEVELOPMENT AND SURVIVAL OF LOW CHILL STONE-FRUIT EMBRYOS RESCUED IN VITRO

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11. EVALUATING MUTATION BREEDING METHODS TO FAST-TRACK THE DOMESTICATION OF TWO AUSTRALIAN NATIVE CALANDRINIA SPECIES FOR ORNAMENTAL HORTICULTURE

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12. PRODUCTION OF SYNTHETIC SEEDS FROM MICRO SHOOTS AND SOMATIC EMBRYOS OF GERBERA JAMESONII BOLUS EX. HOOK F.

R.M. Taha | N.A. Hasbullah | A. Awal

13. IN VITRO CULTURE OF PERESKIA BLEO

N. Jaafar Sidik | M.S. Norihan | K. Shafii

14. IN VITRO FLOWERING AND PROPAGATION OF BULBOPHYLLUM AURICOMUM LINDL., THE ROYAL FLOWER OF MYANMAR

M.M.M. Than | A. Pal | S. Jha

15. ECO-TURF: WATER USE EFFICIENT TURFGRASSES FROM AUSTRALIAN BIODIVERSITY

16. METABOLIC ENGINEERING OF THE MORPHINE PATHWAY IN OPIUM POPPY

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17. CORRELATION BETWEEN BROWNING, TOTAL PHENOLIC CONTENT, POLYPHENOL OXIDASE AND SEVERAL ANTIOXIDATION ENZYMES DURING PISTACHIO TISSUE CULTURE

Pingsheng Leng | Shuchai Su | Fang Wei | Fei Yu | Yunfeng Duan

18. CITRUS GERMPLASM ENHANCEMENT VIA CELL ENGINEERING AND GENETIC ENGINEERING – PROGRESS REPORT

Ji-Hong Liu | Xing-Zheng Fu | Xi-San Huang | Jing Wang | Qi-Jun Fan

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20. APICAL DOMINANCE IN ALSTROEMERIA CULTURED IN VITRO

P. Pumisutapon | R.G.F Visser | G.-J. De Klerk

21. AGROBACTERIUM TUMEFACIENS-MEDIATED TRANSFORMATION OF ONCIDIUM AND ODONTOGLOSSUM WITH THE MUTATED ETHYLENE RECEPTOR ETR1-1

B. Raffeiner | T. Winkelmann | M. Serek

22. BACTERIALLY-INDUCED GROWTH PROMOTION OF MICROPROPAGATED GINGER

E. Rames | S. Hamill | I. Kurtböke

23. IN VIVO FUNCTIONAL ASSAY OF WUS ORTHOLOG IN TOBACCO

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27. TISSUE CULTURE AND SALT STRESS IN CHRYSANTHEMUM MORIFOLIUM

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30. MICROPROPAGATION OF VEGETATIVELY PROPAGATED CROPS: ACCELERATING RELEASE OF NEW CULTIVARS AND PROVIDING AN IMPORTANT SOURCE OF CLEAN PLANTING MATERIAL

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T. Tapingkae | P. Kristiansen | A. Taji

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34. ESTABLISHING METHODS FOR INTER-GENERIC HYBRIDIZATION OF AUSTRALIAN NATIVE CALANDRINIA AND EXOTIC PORTULACA SPECIES FOR ORNAMENTAL HORTICULTURE

P. Wickramasinghe | D.K. Harrison | M.E. Johnston

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Jin-Hu Wu | A.R. Ferguson | B.G. Murray

36. CHARACTERIZATION OF CHLOROSIS-RESISTANT APPLE MUTANTS OF TRANSGENIC FRO2

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R. Honjo | T. Ikeda | A. Taji | A. Uragami

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N. Adak | L. Kaynak | M. Pekmezci | H. Gubbuk

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51. SOMATIC EMBRYOGENESIS AND PRODUCTION OF ARTIFICIAL SEEDS IN SAINTPAULIA IONANTHA WENDL.

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63. EFFECTS OF ELECTRIC CURRENT VALUE AND TIME TREATMENTS ON ELIMINATION OF CARNATION MOTTLE VIRUS AND IN VITRO PLANTLET REGENERATION OF CARNATION (DIANTHUS CARYOPHYLLUS)

S. Sepahpour | A. Moieni | M. Shamsbakhsh | A. Baghizadeh

64. EFFECT OF CHITOSAN AND JASMONIC ACID RELATED COMPOUNDS ON SHOOT FORMATION FROM RHIZOME CULTURES OF AN ORIENTAL CYMBIDIUM SPECIES

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M. Kawaguchi | D. Backhouse | T. Tapingkae | A. Taji

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67. INTERGENERIC HYBRIDIZATION, EMBRYO RESCUE AND MOLECULAR DETECTION FOR INTERGENERIC HYBRIDS BETWEEN ASCOCENDA AND PHALAENOPSIS

C.C. Tsai | Y.C. Chiang | S.C. Huang | W.L. Liu | C.H. Chou

68. GROWTH AND WATER STATUS OF TISSUE-CULTURED STRAWBERRY PLANTS AS AFFECTED BY SALT STRESS AND DIFFERENT SUGAR CONCENTRATION IN MEDIA

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69. ESTABLISHMENT OF IN VITRO SALVIA CELL BIOMASS FOR THE CONTROLLED PRODUCTION OF ANTIOXIDANT METABOLITES