Articles
ESTABLISHMENT OF EMBRYOGENIC CELL SUSPENSION AND FIELD EVALUATION OF REGENERANTS OF ZINGIBER ZERUMBET SMITH
Article number
829_49
Pages
319 – 324
Language
English
Abstract
Zingiber zerumbet Smith, a medicinal ginger containing zerumbone which has been shown to have anti-inflammatory properties and inhibitory activity against Epstein-Barr virus which can cause certain cancers.
Tissue culture technique through embryogenic cell suspension was found to be the best solution to provide disease free planting materials and in abundance.
Explants were cultured on M1 media which consist of MS basal medium supplemented with 1 mg/L biotin, 1 mg/L 2,4-D, 1 mg/L IAA, and 1 mg/L NAA under dark conditions to induce embryogenic callus which were transferred to M2D liquid medium to establish proliferating cell suspension.
Shoots were regenerated on hormone free MS basal media without light for a maximum of 8 weeks and were transferred to a BAP augmented MS media under a light regime to produce complete plants.
Approximately 210 plantlets per ml of the settled cell volume (SCV) were produced.
The regenerants were grown to maturity (10 to 12 months) in the field.
The rhizomes from the tissue culture derived plants were shown to have more tillers and higher rhizome yield than the conventional rhizome propagated plants.
The amount of zerumbone present in tissue cultured derived rhizomes were higher compared to the conventionally propagated plants.
Tissue culture technique through embryogenic cell suspension was found to be the best solution to provide disease free planting materials and in abundance.
Explants were cultured on M1 media which consist of MS basal medium supplemented with 1 mg/L biotin, 1 mg/L 2,4-D, 1 mg/L IAA, and 1 mg/L NAA under dark conditions to induce embryogenic callus which were transferred to M2D liquid medium to establish proliferating cell suspension.
Shoots were regenerated on hormone free MS basal media without light for a maximum of 8 weeks and were transferred to a BAP augmented MS media under a light regime to produce complete plants.
Approximately 210 plantlets per ml of the settled cell volume (SCV) were produced.
The regenerants were grown to maturity (10 to 12 months) in the field.
The rhizomes from the tissue culture derived plants were shown to have more tillers and higher rhizome yield than the conventional rhizome propagated plants.
The amount of zerumbone present in tissue cultured derived rhizomes were higher compared to the conventionally propagated plants.
Authors
F.D. Idris, M. Jalil, H. Ibrahim, N. Khalid
Keywords
embryogenic, cell suspension, zerumbone, field evaluation, Zingiber zerumbet
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