Articles
EFFECT OF CHITOSAN AND JASMONIC ACID RELATED COMPOUNDS ON SHOOT FORMATION FROM RHIZOME CULTURES OF AN ORIENTAL CYMBIDIUM SPECIES
Article number
829_64
Pages
399 – 402
Language
English
Abstract
We examined the effect of a jasmonic acid related compound (n-propyl dihydrojasmonate; PDJ), methl jasmonate (Me-JA), and chitosan H added to modified MS medium on shoot formation of a Cymbidium species (C. kanran Makino). Single addition of Me-JA was effective for shoot formation from rhizome cultures.
Combination treatments of chitosan H with Me-JA were also effective for shoot formation from rhizome cultures.
Percentage of rooting was highest (64.3%) in a combination treatment of 1 mg L-1 chitosan H + 0.01 µM Me-JA. Single addition of PDJ to culture medium was effective for shoot formation from rhizome cultures, and showed a higher number and rate at 0.1 µM PDJ. Combinations of Chitosan H with PDJ were more effective for promoting shoot formation from rhizome cultures.
The addition of PDJ in combination with 1 mg L-1 chitosan H was most effective for shoot formation.
Rooting from newly formed shoots was promoted by addition of 1 mg L-1 chitosan H. The percentage of rooting was higher (60%) in the combination treatment of 1 mg L-1 chitosan H with 0.01 µM PDJ. These results indicate that a combination of jasmonic acid derivatives and chitosan has a significant effect on both shoot and root formation from cultures of oriental Cymbidium species (C. kanran).
Combination treatments of chitosan H with Me-JA were also effective for shoot formation from rhizome cultures.
Percentage of rooting was highest (64.3%) in a combination treatment of 1 mg L-1 chitosan H + 0.01 µM Me-JA. Single addition of PDJ to culture medium was effective for shoot formation from rhizome cultures, and showed a higher number and rate at 0.1 µM PDJ. Combinations of Chitosan H with PDJ were more effective for promoting shoot formation from rhizome cultures.
The addition of PDJ in combination with 1 mg L-1 chitosan H was most effective for shoot formation.
Rooting from newly formed shoots was promoted by addition of 1 mg L-1 chitosan H. The percentage of rooting was higher (60%) in the combination treatment of 1 mg L-1 chitosan H with 0.01 µM PDJ. These results indicate that a combination of jasmonic acid derivatives and chitosan has a significant effect on both shoot and root formation from cultures of oriental Cymbidium species (C. kanran).
Authors
K. Shimasaki, T. Wang, Y. Nishimura, N.T. Huyen, S. Neera, T. Hsiung, C. Huang
Keywords
Cymbidium, rhizome, chitosan, jasmonic acid derivatives
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