VARIETAL IDENTIFICATION AND GENETIC PURITY ASSESSMENT OF F₁ HYBRID SEEDS USING RAPD MARKERS IN FABA BEAN (VICIA FABA L.)

Random amplified polymorphic DNA (RAPD) markers were used to distinguish between several faba bean (Vicia faba L.) genotypes, comprising eight inbred lines and 28 F1 hybrids derived from eight parents.
Amplification conditions and reproducibility of RAPD patterns were examined.
Comparison of polymerase chain reaction (PCR) products obtained using 25 decamer arbitrary primers allowed identification of all the lines analyzed.
With several primers, line specific RAPD markers were identified, while with others, polymorphism was more extensive, revealing several RAPD markers for several lines.
Because of the Mendelian segregation of these molecular markers, they were applied to evaluate the genetic purity of F₁ hybrid seeds.
In RAPD analysis, nineteen primers produced a total of 149 bands, out of which 128 bands (86.9%) were found to be polymorphic.
The pair-wise differences detected by RAPD-PCR analysis were used to calculate the genetic distance and to construct a dendrogram among the tested lines and their parents.
Very low genetic distance was found between two varieties ‘Sakha 1’ and ‘Giza 402’.

VI International Symposium on In Vitro Culture and Horticultural Breeding

S.S. Alghamdi

faba bean, varietal identification, seed samples purity, RAPD markers, genetic distance

https://doi.org/10.17660/ActaHortic.2009.829.39