Articles
SLOW GROWTH IN VITRO CONSERVATION OF DENDROBIUM DRACONIS RCHB. F.: EFFECT OF BLACK KWAO KRUA (MUCUNA COLLETTII LACE)
Article number
829_56
Pages
355 – 358
Language
English
Abstract
A slow growth system for in vitro conservation of Dendrobium draconis Rchb. f. was developed.
Seeds were cultured on Vacin and Went medium supplemented with 200 ml/L of coconut water (VW). Three-month-old protocorms were transferred to slow growth medium, which was modified by adding powder from tuberous roots of black Kwao Krua (Mucuna collettii). The modified medium was a VW medium containing Kwao Krua powder at various concentrations (0, 5, 10, 15, and 20 mg/L). The cultures were stored at 25°C under a 12-h photoperiod (cool white fluorescent lamps, approx. 20 µmol m-2s-1 light intensity) for six months without an intervening subculture.
Storage of a microplants of Dendrobium draconis in the absence of Kwao Krua powder was not feasible with subculture intervals of more than three months.
The results showed the shoot culture in slow growth medium could be maintained satisfactorily without subculture.
The average height of microplants in each concentration varied from 3.3 mm to 3.8 mm, while the average height of microplants on normal medium was 17.8 mm.
After six months of storage, the microplants were transferred to fresh normal VW medium and rapidly developed normal healthy shoots within three months.
Seeds were cultured on Vacin and Went medium supplemented with 200 ml/L of coconut water (VW). Three-month-old protocorms were transferred to slow growth medium, which was modified by adding powder from tuberous roots of black Kwao Krua (Mucuna collettii). The modified medium was a VW medium containing Kwao Krua powder at various concentrations (0, 5, 10, 15, and 20 mg/L). The cultures were stored at 25°C under a 12-h photoperiod (cool white fluorescent lamps, approx. 20 µmol m-2s-1 light intensity) for six months without an intervening subculture.
Storage of a microplants of Dendrobium draconis in the absence of Kwao Krua powder was not feasible with subculture intervals of more than three months.
The results showed the shoot culture in slow growth medium could be maintained satisfactorily without subculture.
The average height of microplants in each concentration varied from 3.3 mm to 3.8 mm, while the average height of microplants on normal medium was 17.8 mm.
After six months of storage, the microplants were transferred to fresh normal VW medium and rapidly developed normal healthy shoots within three months.
Authors
N. Rangsayatorn, K. Jina, S. Pampasit
Keywords
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