Articles
INVESTIGATING THE IN VITRO GROWTH INHIBITION OF ORYZALIN-TREATED GERBERA JAMESONII
Article number
829_47
Pages
309 – 312
Language
English
Abstract
Some herbicide agents such as oryzalin are widely used for in vitro polyploidization due to their depolymerization effects on microtubules, which usually direct the polar distribution of sub-cellular components.
Side effects of the ployploidisation process occur since these herbicides hinder plant growth by inhibiting cell division and elongation.
To determine dose rates for ployploidisation that would least affect plant development, the effect of oryzalin on the in vitro growth of Gerbera jamesonii Red explosion was examined.
Five concentrations of oryzalin (0, 30, 60, 120, and 240 µM) in liquid medium and three exposure periods (12, 24, and 48 hours) were evaluated.
After treatment, plantlets were placed in solid Murashigue and Skoog medium with three concentrations of kinetin (2, 4, and 6 mg L-1). The number of new leaves produced and fresh weight of cultured plantlets were used to determine the growth inhibition effects of oryzalin.
Although in all of the treatments, there was not a significant difference in the number of new leaves and fresh weight of the treated plantlets, the results illustrated that as the concentration of oryzalin or the exposure period was increased, the growth rate was deceased.
Also in the control treatment of all of the exposure periods, growth rate was significantly higher when 2 mg/L and 4 mg/L kinetin were used compared to the treatment of 6 mg/L.
Side effects of the ployploidisation process occur since these herbicides hinder plant growth by inhibiting cell division and elongation.
To determine dose rates for ployploidisation that would least affect plant development, the effect of oryzalin on the in vitro growth of Gerbera jamesonii Red explosion was examined.
Five concentrations of oryzalin (0, 30, 60, 120, and 240 µM) in liquid medium and three exposure periods (12, 24, and 48 hours) were evaluated.
After treatment, plantlets were placed in solid Murashigue and Skoog medium with three concentrations of kinetin (2, 4, and 6 mg L-1). The number of new leaves produced and fresh weight of cultured plantlets were used to determine the growth inhibition effects of oryzalin.
Although in all of the treatments, there was not a significant difference in the number of new leaves and fresh weight of the treated plantlets, the results illustrated that as the concentration of oryzalin or the exposure period was increased, the growth rate was deceased.
Also in the control treatment of all of the exposure periods, growth rate was significantly higher when 2 mg/L and 4 mg/L kinetin were used compared to the treatment of 6 mg/L.
Authors
Z. Ghayoor Karimiani, A. Bagheri, G.H. Davarynejad, M. Jafarkhani Kermani
Keywords
Gerbera jamesonii, kinetin, growth rate, oryzalin
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