Articles
A DECONTAMINATION PROCEDURE FOR IN VITRO CULTURE OF LILIUM LONGIFLORUM CV. ‘DOZZEL’ SCALE EXPLANTS
Article number
829_43
Pages
289 – 293
Language
English
Abstract
Many species of lily such as Lilium longiflorum cv. Dozzel are of considerable value in the cut-flower and potted-plant markets.
As tissue culture has become an increasingly popular method for the multiplication and distribution of lilies, the need for an effective method of decontaminating initial explant material is required.
Currently, methods to decontaminate scale explants result in high levels of contamination.
Therefore we compared several decontamination treatments for use in in vitro culture of Lilium longiflorum cv. Dozzel. Bulbs of lily were selected from a hydroponic greenhouse in Kordan (Tehran, Iran), which had produced one flower in the current season.
After the initial external cleaning, bulbs were scaled and kept in Tween20 for 30 seconds, and then washed in running tap water for one hour.
Treatments carried out before culture were either: 1) Hot water treatment at 45°C for 1 h; 2) Hot water treatment at 55°C for 1 h; 3) Hot water treatment at 65°C for 1 h; 4) Hot water treatment at 55°c for 1 h + 70% ethanol for 1 min; 5) Hot water treatment at 45°C for 1 h + 70% ethanol for 1 min; 6) 1% Captan fungicide for 1 h + 2% sodium hypochlorite solution for 20 min; 7) 2% sodium hypochlorite solution for 10 min + 70% ethanol for 1 min + 0.1% HgCl2 for 7 min; 8) 2% sodium hypochlorite solution for 10 min + 70% ethanol for 1 min + 0.1% HgCl2 for 5 min; 9) 2% sodium hypochlorite solution for 15 min + 70% ethanol for 1 min + 0.1% HgCl2 for 5 min; 10) 2% sodium hypochlorite solution for 20 min + 70% ethanol for 1 min + 0/1% HgCl2 for 5 min.
The best results were obtained from the 9th treatment with contamination of less than 15% and greatest survival.
As tissue culture has become an increasingly popular method for the multiplication and distribution of lilies, the need for an effective method of decontaminating initial explant material is required.
Currently, methods to decontaminate scale explants result in high levels of contamination.
Therefore we compared several decontamination treatments for use in in vitro culture of Lilium longiflorum cv. Dozzel. Bulbs of lily were selected from a hydroponic greenhouse in Kordan (Tehran, Iran), which had produced one flower in the current season.
After the initial external cleaning, bulbs were scaled and kept in Tween20 for 30 seconds, and then washed in running tap water for one hour.
Treatments carried out before culture were either: 1) Hot water treatment at 45°C for 1 h; 2) Hot water treatment at 55°C for 1 h; 3) Hot water treatment at 65°C for 1 h; 4) Hot water treatment at 55°c for 1 h + 70% ethanol for 1 min; 5) Hot water treatment at 45°C for 1 h + 70% ethanol for 1 min; 6) 1% Captan fungicide for 1 h + 2% sodium hypochlorite solution for 20 min; 7) 2% sodium hypochlorite solution for 10 min + 70% ethanol for 1 min + 0.1% HgCl2 for 7 min; 8) 2% sodium hypochlorite solution for 10 min + 70% ethanol for 1 min + 0.1% HgCl2 for 5 min; 9) 2% sodium hypochlorite solution for 15 min + 70% ethanol for 1 min + 0.1% HgCl2 for 5 min; 10) 2% sodium hypochlorite solution for 20 min + 70% ethanol for 1 min + 0/1% HgCl2 for 5 min.
The best results were obtained from the 9th treatment with contamination of less than 15% and greatest survival.
Authors
B. Ghoreyshi, R. Naderi, S.A. Ghaem Maghami, S. Zeyni
Keywords
decontamination, lilium, scale, HgCl2, sodium hypochlorite
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