Articles
SOMATIC EMBRYOGENESIS IN CHESTNUT
Article number
280_27
Pages
159 – 162
Language
Abstract
We have evaluated the use of cotyledons as starting material to induce somatic embryogenesis in two cultivars of Castanea sativa and of two hybrid cultivars of C. crenata x C. sativa.
Somatic embryos were obtained, in darkness, only from immature ‘G9’ cotyledons cultured on half-strength Murashige-Skoog (½ MS) medium (1962) supplemented either with NAA alone or 2,4-D plus BA but not with B5 medium (Gamborg et al., 1968). Somatic embryos developed slightly when transferred to MS plus BA, GA3 and IBA, but well-developed seedlings were not observed.
Authors
C. Piagnani, T. Eccher
Keywords
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