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Articles

CELL ENGINEERING BY PROTOPLAST FUSION: IMPLICATIONS FOR PLANT IMPROVEMENT

Article number

280_39

Pages

227 – 232

Language

Abstract

The transfer of cytoplasmic genomes from Lycopersicon and Solanum species to L. esculentum was attempted by means of the "donor-recipient" method.
A cytoplasmic-albino Large Red Cherry mutant (ALRC) was utilized as the recipient genotype.
Protoplasts from cytoplasm-donor genotypes were irradiated with X-rays, in order to induce chromosome elimination and transfer of cytoplasmic genophores upon fusion.

Putative cybrid colonies were isolated and subsequently regenerated.
Characterization of ALRC x L. peruvianum cybrids revealed isozyme patterns identical to L. esculentum.. Cybrid plants exhibit a recombinant mt-DNA pattern, containing parental as well as novel fragments.

Publication

I International Symposium on In Vitro Culture and Horticultural Breeding

Authors

A. Zelcer, S. Izhar, D. Wolf, B. Levy, O. Soferman

Keywords

Full text

https://doi.org/10.17660/ActaHortic.1990.280.39

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