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Articles

TOWARDS GENETIC TRANSFORMATION OF BEAN BY AGROBACTERIUM TUMEFACIENS.

Article number

280_87

Pages

527 – 536

Language

Abstract

The relative ability of different bean genotypes to produce tumours following inoculation with several wild-type strains of Agrobacterium was determined both in vivo and in vitro.
The cultivars tested were susceptible to most of the bacterial strains, but a genotype specific response for both the frequency and the size of induced tumours was observed.
Engineered strains carrying a kanamycin resistance determinant were used for leaf disk cocultivation experiments.
Callus tissues, which developed from leaf disk cut edges, were able to grow for several subcultures onto antibiotic-containing medium.
The transformed nature of these calli was confirmed by assaying neomycin phosphotransferase (NPTII) activity.

Publication

I International Symposium on In Vitro Culture and Horticultural Breeding

Authors

A. Genga, A. Allavena, A. Ceriotti, R. Bollini

Keywords

Full text

https://doi.org/10.17660/ActaHortic.1990.280.87

Online Articles (95)

2. ORGANOGENESIS AND EMBRYOGENESIS: GENETIC AND PHYSIOLOGICAL APPROACHES

V. Nuti Ronchi

3. TISSUE CULTURE AND THE GENETIC IMPROVEMENT OF GRAPEVINES: A REVIEW

M. G. Mullins

4. SYNTHETIC SEED TECHNOLOGY: IMPROVING DESICCATION TOLERANCE OF SOMATIC EMBRYOS OF CELERY

Y. Kim | J. Janick

5. BASIC PEROXIDASES AND ROOTING IN MICROCUTTINGS OF MALUS

G. Klerk | J. Brugge | R. Smulders | M. Benschop

6. LEAF-CALLUS GROWTH, SHOOT REGENERATION AND SOMACLONAL VARIATION IN ACTINIDIA DELICIOSA : EFFECT OF MEDIA PH

G. Marino | S. Battistini

7. THE USE OF A BRIEF 2,4-D TREATMENT TO INDUCE LEAF REGENERATION ON PRUNUS CANESCENS BOIS

M. Antonelli | Ph. Druart

8. IMPROVING YIELD OF ADVENTITIOUS SHOOTS IN APPLE

M. Dufour

9. CULTIVAR DEPENDENT RESPONSES TO REGENERATION FROM LEAVES IN APPLE

F. Fasolo Fabbri Malavasi | S. Predieri

10. FACTORS INFLUENCING SOMATIC EMBRYOGENESIS AND PLANTLET REGENERATION IN PECAN, CARYA ILLINOENSIS

Hazel Y. Wetzstein | James R. Ault | Scott A. Merkle

11. REGULATION OF LARGE SCALE EMBRYOGENESIS IN CELERY

B.L. Nadel | A. Altman | M. Ziv

12. ORGANOGENESIS AND EMBRYOGENESIS IN LISIANTHUS RUSSELLIANUS HOOK

B. Ruffoni | C. Damiano | F. Massabò | P. Esposito

13. ABSCISIC ACID INDUCED HIGH FREQUENCY EMBRYOGENESIS FROM SAPINDUS TRIFOLIATUS LEAVES

S.K. Unnikrishnan | A.R. Mehta | P.N. Bhatt

14. ADVENTITIOUS SHOOT FORMATION FROM IN VITRO LEAVES OF MM.106 APPLE CLONAL ROOTSTOCK

M. ANCHERANI | P. ROSATI | S. PREDIERI

15. TISSUE CULTURES OF BEAN (P. COCCINEUS L.) AND THEIR APPLICATIONS TO BREEDING

R. Angelini Rota | A. Genga | A. Allavena

16. IN VITRO SHOOT REGENERATION IN PASSIFLORA COERULEA

F. Vestri | S. Schiff | A. Bennici

17. ESTABLISHMENT OF AN IN VITRO RIBES GERMPLASM COLLECTION AND PRELIMINARY INVESTIGATIONS INTO LONG-TERM LOW TEMPERATURE GERMPLASM STORAGE

R.M. Brennan | S. Millam | D. Davidson | A. Wilshin

18. INDUCTION OF SOMATIC EMBRYOGENESIS IN GENISTA MONOSPERMA LAM.

P. Curir | B. Ruffoni | F. Massabò | C. Damiano

19. EFFECT OF CULTURE CONDITIONS AND LEAF SELECTION ON ORGANOGENESIS OF MALUS DOMESTICA CV. MCINTOSH “WIJCIK” AND PRUNUS CANESCENS BOIS GM79.

Ph. Druart

20. IMPROVEMENT OF SOMATIC EMBRYOGENESIS OF THE CHERRY DWARF ROOTSTOCK INMIL/GM9 BY THE USE OF DIFFERENT CARBON SOURCES

Ph. Druart

21. COMPARATIVE STUDIES ON IN VITRO REGENERATION OF SEEDLING EXPLANTS IN CHILI PEPPER (CAPSICUM ANNUUM L.)

M. Fári | Z. Túry | Mrs. F. Csillag

22. REGENERATION FROM ALSTROEMERIA CALLUS

E. Gonzalez Benito | P.G. Alderson

23. CALLUS FORMATION FROM TOMATO ENDOSPERM

V. Kagan-Zur | D. Mills | Y. Mizrahi

24. PROPAGATION AND ORGANOGENESIS “IN VITRO” OF ANACARDIUM OCCIDENTALE L.

A.R. Leva | A.M. Falcone

25. IN VITRO CLONAL PROPAGATION OF STRAWBERRY FROM IMMATURE ACHENES

E.K. Lis

26. ETHYLENE INVOLVEMENT IN IN VITRO REGENERATION OF LILY

A. Mensuali Sodi | M. Panizza | F. Tognoni

27. EFFECT OF LIGHT QUALITY ON REGENERATION FROM CALLUS OF ACTINIDIA DELICIOSA

R. Muleo | S. Morini

28. SOMATIC EMBRYOGENESIS IN CHESTNUT

C. Piagnani | T. Eccher

29. IN VITRO CULTURE TECHNIQUES FOR CARNATION BREEDING

L. Radojevic | N. Djordjevic | J. Petrovic

30. REGULATION OF ORGANOGENESIS WITH JASMONIC ACID

M. Ravnikar | J. Rode | N. Gogala | D. Benedicic

31. ADVENTIVE REGENERATION IN CELL COLONIES OF CARNATION

B. Ruffoni | F. Massabò | P. Curir | C. Guda

32. FIELD EVALUATION OF ANDROGENETIC LINES OF EGGPLANT

M.C. Sanguineti | R. Tuberosa | S. Conti

33. LONG-TERM SOMATIC EMBRYO PRODUCTION AND PLANT REGENERATION FROM EMBRYO-DERIVED PEACH CALLUS

R. Scorza | J.M. Cordts | S. Mante

34. EFFECT OF CYTOKININS ON EMBRYOGENIC CALLUS FORMATION FROM UNDEVELOPED OVULES OF ORANGE

A. Starrantino | P. Caponnetto

35. INFLUENCE OF TDZ AND BA ON ADVENTITIOUS SHOOT REGENERATION FROM APPLE LEAVES

C. Theiler-Hedtrich | R. Theiler-Hedtrich

36. ORGANOGENESIS AND SOMATIC EMBRYOGENESIS FROM CALLUS OF WINGED BEAN (PSOPHOCARPUS TETRAGONOLOBUS (L.) DC.)

S. Venketeswaran | M.A.D.L. Dias | Ursula V. Weyers

37. ORGANOGENESIS AND SOMATIC EMBRYOGENESIS FROM CALLUS OF WINGED BEAN (PSOPHOCARPUS TETRAGONOLOBUS (L.) DC.)

S. Venketeswaran | M.A.D.L. Dias | Ursula V. Weyers

38. THE EFFECT OF GROWTH RETARDANTS ON SHOOT PROLIFERATION AND MORPHOGENESIS IN LIQUID CULTURED GLADIOLUS PLANTS

M. Ziv

39. PROTOPLAST TECHNOLOGY AND TOP-FRUIT TREE BREEDING

Sergio J. Ochatt

40. CELL ENGINEERING BY PROTOPLAST FUSION: IMPLICATIONS FOR PLANT IMPROVEMENT

A. Zelcer | S. Izhar | D. Wolf | B. Levy | O. Soferman

41. NON-AXENIC PLANT TISSUE CULTURE: POSSIBILITIES AND OPPORTUNITIES

Edwin B. Herman

42. CITRUS CYBRIDS: TRANSFER OF MICROCITRUS ORGANELLES INTO CITRUS CULTIVARS

A. Vardi | A. Frydman-Shani | E. Galun | P. Gonen | S. Bliechman

43. USE OF PROTOPLAST, CELL, AND SHOOT TIP CULTURE IN AN ELM GERMPLASM IMPROVEMENT PROGRAM

44. PROTOPLAST ISOLATION AND PLANT REGENERATION IN ORNAMENTAL COMPOSITAE

J.S. Al-Atabee | J.B. Power

45. POLYAMINE UPTAKE IN PROTOPLASTS AND PLANT ORGANELLES

N. Bagni | R. Pistocchi

46. EVALUATION OF ATRAZINE RESISTANCE IN PROTOPLAST REGENERANTS OF SOLANUM NIGRUM L..

M. De Donato | C. Mozzetti | E. Perucco

47. CALLUS AND CELL CULTURE IN SUGARBEET

E. Forti | G. Mandolino | P. Ranalli

48. FLOW CYTOMETRIC ASSESSMENT OF VIABILITY AND DIFFERENTIATION IN POTATO AND ORANGE CELL SUSPENSION PROTOPLASTS.

49. ISOLATION AND CULTURE OF MESOPHYLL PROTOPLASTS OF GRAPE (VITIS VINIFERA L.), CV. TREBBIANO DI ROMAGNA

G. Marino

50. ADVANCES IN PLANT REGENERATION FROM APPLE PROTOPLASTS

Estela M. Patat-Ochatt | John B. Power

51. INFLUENCE OF LIGHT INTENSITY ON TOMATO CALLUS PRODUCTION

A.C. Wetten | I.P. Cumbus

52. INDUCTION OF HAPLOIDY IN FRUIT TREES

Y.X. Zhang | Y. Lespinasse | E. Chevreau

53. EMBRYOGENESIS, ORGANOGENESIS AND PLANT REGENERATION FROM ANTHER CULTURE IN VITIS

A. Cersosimo | M. Crespan | G. Paludetti | M.M. Altamura

54. ATTEMPTS TO REGENERATE HAPLOID PLANTS FROM “IN VITRO” CULTURES OF CITRUS ANTHERS

G. Geraci | A. Starrantino

55. MEIOSIS AND FERTILITY IN ANTHER-CULTURE-DERIVED CABBAGE 2X-PLANTS

M.S. Chiang | R. Crête | S. Fréchette | C. Chong

56. ANTHER CULTURE OF WINTER-HEADING CAULIFLOWER

M.P. Fuller | S. Turton | B.W.W. Grout

57. INDUCTION OF ANDROGENESIS IN VITRO IN APPLE AND SWEET CHERRY

M. Hoefer | V. Hanke

58. GENOTYPE RESPONSE TO IN VITRO GYNOGENESIS TECHNIQUE IN GERBERA JAMESONII

A. Tosca | M. Lombardi | L. Marinoni | L. Conti | P. Frangi

59. CELLULAR MECHANISMS OF SALT AND WATER STRESS TOLERANCE IN PLANTS

60. SOMACLONAL VARIATION AND CHEMICALLY INDUCED MUTAGENESIS FOR THE PRODUCTION OF SALT AND DROUGHT TOLERANCE IN TOMATO

61. ACTIVE DEFENSE PROCESSES AND IN VITRO SELECTION FOR RESISTANCE TO PATHOGENS

62. INDUCTION OF DWARF F-12/1 CHERRY ROOTSTOCKS BY IN VITRO MUTAGENESIS

R. Theiler-Hedtrich

63. INHERITANCE AND STABILITY OF SOMACLONAL VARIATIONS IN MICROPROPAGATED STRAWBERRY

S. Sansavini | P. Rosati | D. Gaggioli | M.F. Toschi

64. HISTOLOGICAL AND CYTOLOCICAL SELECTION OF SOMACLONAL AND INDUCED VARIANTS OF ASPARAGUS DERIVED FROM CALLUS CULTURES

G. Reuther

65. SOMATIC MUTAGENESIS OF GERBERA JAMESONII HYBR.: IRRADIATION AND IN VITRO CULTURE

U. Laneri | R. Franconi | P. Altavista

66. SOMACLONAL VARIATION IN PEACH: SCREENING FOR RESISTANCE TO XANTHOMONAS CAMPESTRIS PV. PRUNI AND PSEUDOMONAS SYRINGAE PV. SYRINGAE

F.A. Hammerschlag | V. Ognjanov

67. IN VITRO SELECTION OF APPLE ROOTSTOCK SOMACLONES WITH PHYTOPHTHORA CACTORUM CULTURE FILTRATE

68. IMPACT OF OSMOTIC POTENTIAL ON IN VITRO CULTURES OF APPLE

C. Lankes | R. H. Zimmerman

69. SELECTION FOR SALT TOLERANCE IN REGENERATING CULTURES OF COLEUS BLUMEI

K.M. Ibrahim | H.A. Collin | J.C. Collins

70. IN VITRO SELECTION FOR SALT TOLERANCE IN COLEUS BLUMEI

J.C. Collins | K.M. Ibrahim | H.A. Collin

71. TOMATO MUTANTS INDUCED BY IN VITRO CULTURED COTYLEDONS

P. Crino | A. Lai | R. Di Bonito | F. Saccardo

72. SELECTIVE ACTION OF ATRAZINE IN CALLUS CULTURES OF SOLANUM NIGRUM AND NICOTIANA TABACUM

M. De Donato | C. Mozzetti | E. Perucco

73. ETHYLENE PRODUCTION IN TISSUE CULTURE OF PEACH X ALMOND HYBRID, TOMATO, SWEET CHERRY, AND GRAPE

R. Jona | I. Gribaudo

74. A SOMACLONAL VARIANT IN CYMBIDIUM

U. Laneri

75. IN VITRO CULTURE OF CUCUMIS SATIVUS XI. SELECTION OF RESISTANCE TO FUSARIUM OXYSPORUM

S. MALEPSZY | A. EL-KAZZAZ

76. IN VITRO GROWTH PATTERN OF SALT-STRESSED CELLS OF LAVANDIN

A. Mensuali Sodi | G. Serra | C. Vitagliano | F. Blando

77. IN VITRO PROPAGATION OF LAVANDIN: MORPHOLOGICAL CHANGES IN REGENERATED PLANTS

M. Panizza | F. Tognoni | A. Mensuali Sodi

78. EFFECT OF PHOMA TRACHEIPHILA TOXINS ON CITRUS PROTOPLASTS

F. Sesto | V. Grimaldi | A.M. Pennisi

79. INDUCTION AND USE OF VARIATION IN POTATO WITH DIFFERENT IN VITRO SYSTEMS

80. PRODUCTION AND STUDIES OF FERTILE INTERGENERIC HYBRIDS LYCOPERSICON ESCULENTUM MILL. X SOLANUM LYCOPERSICOIDES DUN

P.K. Stoeva | Z.V. Achkova | N.P. Zagorska

81. ION LEAKAGE IN THE PRESENCE OF FUNGAL TOXINS AS A METHOD FOR SCREENING RESISTANT GENOTYPES IN TOMATO AND CARNATION

82. FACTORS INFLUENCING VIRULENCE IN AGROBACTERIUM-MEDIATED TRANSFORMATION OF APPLE

A.M. Dandekar | S.L. Uratsu | N. Matsuta

83. AGROBACTERIUM-MEDIATED TRANSFORMATION OF APPLE AND STRAWBERRY USING DISARMED TI-BINARY VECTORS

D.J. James | A.J. Passey | D.J. Barbara

84. THE POTENTIAL FOR GENETIC MANIPULATION OF PEACH FRUIT DEVELOPMENT AND QUALITY THROUGH DIRECTED GENE TRANSFER

85. LIPOSOME-MEDIATED INTRODUCTION OF DNA INTO PLANT PROTOPLASTS AND CALLI

86. PLANTLET REGENERATION AND GENETIC TRANSFORMATION IN SOFT FRUIT SPECIES

J. Graham | Ronald J. McNicol

87. USE OF HYPERVIRULENT AGROBACTERIUM TUMEFACIENS CARRYING A BINARY VECTOR TO TRANSFORM EGGPLANT (SOLANUM MELONGENA L.) COTYLEDONARY LEAF DISCS.

E. Filippone | Paul F. Lurquin

88. TOWARDS GENETIC TRANSFORMATION OF BEAN BY AGROBACTERIUM TUMEFACIENS.

A. Genga | A. Allavena | A. Ceriotti | R. Bollini

89. RESISTANCE TO BACTERIAL DISEASE: PRELIMINARY RESULTS OF A BIOTECHNOLOGICAL APPROACH IN POTATO

C. MONTANELLI | G. NASCARI

90. THE POTENTIAL USE OF RESTRICTION FRAGMENT LENGTH POLYMORPHISM IN RUBUS BREEDING

R. Waugh | M. van de Van | S. Millam | R. Brennan | W. Powell

91. MOLECULAR PHENOGENETICS AS AN AID TO FRUIT BREEDING

Don J. Durzan

92. ISOLATION, CHARACTERIZATION AND EXPRESSION OF TOMATO SUPEROXIDE DISMUTASE GENES – AN UPDATE AND COMPARISON WITH SIMILAR GENES FROM OTHER PLANTS

E. Galun | R. Perl-Treves | B. Nacmias | N. Magal | D. Aviv

93. IN VITRO FLOWER DEVELOPMENT OF THE NORMAL AND A MALE STERILE, STAMENLESS-2 MUTANT OF TOMATO (LYCOPERSICON ESCULENTUM MILL.)

V.K. Sawhney | R. Rastogi

94. GENE EXPRESSION AND EPIPHYLLOUS BUD REACTIVATION IN KALANCHOE MORTAGEI

Y.T. JASRAI | S. UNNIKRISHNAN | P.N. BHATT | A.R. MEHTA

95. EXPRESSION OF THE PLUM POX VIRUS COAT PROTEIN GENE IN NICOTIANA CLEVELANDII