Articles
SOMACLONAL VARIATION AND CHEMICALLY INDUCED MUTAGENESIS FOR THE PRODUCTION OF SALT AND DROUGHT TOLERANCE IN TOMATO
Article number
280_59
Pages
353 – 360
Language
Abstract
Mutagenized populations of tomato (Lycopersicon esculentum Mill.) were screened for tolerance to salinity and drought.
Selection was performed on the R2 or M2 plantlets, i.e. on the offspring of regenerated plants or of plants obtained from mutagenized (EMS or MNU) seed.
For salt tolerance, 113 R2 and 134 M2 families were screened for their capacity to grow in the presence of 1.8% NaCl.
Nine families from both mutant classes survived the treatment.
Progenies of these were challenged with 2.5% NaCl.
Plants from surviving families were grown to maturity and their progeny (fourth generation) used in field trials on salty land.
A good correlation was found between salt tolerance in the laboratory and tolerance in the field.
In the case of drought resistance, 85 R2 and 458 M2 families were grown in the presence of 30% polyethylene glycol.
Three R2 and five M2 families survived the treatment.
Progeny of surviving plants was again selected in the greenhouse under severe water stress conditions: two (one from the R and the other from the M population) were confirmed as true mutants.
Under severe stress conditions proline accumulation in detached leaves was considerally reduced as compared to non-tolerant plants.
Selection was performed on the R2 or M2 plantlets, i.e. on the offspring of regenerated plants or of plants obtained from mutagenized (EMS or MNU) seed.
For salt tolerance, 113 R2 and 134 M2 families were screened for their capacity to grow in the presence of 1.8% NaCl.
Nine families from both mutant classes survived the treatment.
Progenies of these were challenged with 2.5% NaCl.
Plants from surviving families were grown to maturity and their progeny (fourth generation) used in field trials on salty land.
A good correlation was found between salt tolerance in the laboratory and tolerance in the field.
In the case of drought resistance, 85 R2 and 458 M2 families were grown in the presence of 30% polyethylene glycol.
Three R2 and five M2 families survived the treatment.
Progeny of surviving plants was again selected in the greenhouse under severe water stress conditions: two (one from the R and the other from the M population) were confirmed as true mutants.
Under severe stress conditions proline accumulation in detached leaves was considerally reduced as compared to non-tolerant plants.
Authors
F. Sala, G. Baratta, G. Todesco, G. Consonni, G. Gavazzi, C. Tonelli, F. Vecchio
Keywords
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