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EXPRESSION OF THE PLUM POX VIRUS COAT PROTEIN GENE IN NICOTIANA CLEVELANDII

Article number

280_94

Pages

577 – 580

Language

Abstract

Attempts by genetic engineering techniques are made in breeding for virus resistance.
The expression of the viral coat protein in transgenic plants has been shown to induce tolerance against virus infection.
The molecular basis of this phenomenon is related to cross protection induced by mild strains of viruses.

We have chosen the Plum Pox Virus (PPV) as a model.
The viral coat protein gene (Mattanovich et al., 1988) was set under control of the CaMV 35S promoter and introduced into an Agrobacterium tumefaciens binary vector.

Different explants from Nicotiana clevelandii were incubated with Agrobacterium tumefaciens suspensions and transformed shoots were regenerated on media containing kanamycin.
Recombinant Plum Pox Virus coat protein was identified by immuno western blot.

Publication

I International Symposium on In Vitro Culture and Horticultural Breeding

Authors

M. Laimer da Câmara Machado, A. da Câmara Machado, D. Mattanovich, F. Regner, V. Hanzer, B. Durniok, H. Steinkellner, G. Himmler, H. Katinger

Keywords

Full text

https://doi.org/10.17660/ActaHortic.1990.280.94

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