Articles
SOMACLONAL VARIATION IN PEACH: SCREENING FOR RESISTANCE TO XANTHOMONAS CAMPESTRIS PV. PRUNI AND PSEUDOMONAS SYRINGAE PV. SYRINGAE
Article number
280_65
Pages
403 – 408
Language
Abstract
Screening for somaclonal variation is a tissue culture technique that has been used to obtain disease resistant plants.
Studies were undertaken to screen for somaclonal variants of peach having resistance to Xanthomonas campestris pv. pruni, causal agent of bacterial spot and Pseudomonas syringae pv. syringae, causal agent of bacterial canker.
A detached-leaf bioassay was used to evaluate peach regenerants derived from ‘Sunhigh’ (susceptible to leaf spot and bacterial canker) and ‘Redhaven’ (moderately resistant to leaf spot) embryo callus cultures.
Twenty-six clones derived from calli produced on two ‘Sunhigh’ embryos, #61 and #156, and 19 clones derived from calli produced on three ‘Redhaven’ embryos, #30, #46 and #122 were evaluated.
A significantly greater number of clones with levels of leaf spot resistance greater than that found in both ‘Sunhigh’ and ‘Redhaven’ were obtained from calli derived from embryos #156 and #122. Sixty-four percent of the #156-clones and 33% of the #122-clones were more spot resistant than ‘Sunhigh’ and 9% of the #156-clones and 33% of #122-clones were more resistant than ‘Redhaven’. Of the ‘Sunhigh’ and ‘Redhaven’ seedlings screened, 22% of the ‘Sunhigh’ seedlings were more leaf spot resistant than ‘Sunhigh’ and 3% more resistant than ‘Redhaven’. None of the ‘Redhaven’ seedlings was more résistant than ‘Redhaven’.
Studies were undertaken to screen for somaclonal variants of peach having resistance to Xanthomonas campestris pv. pruni, causal agent of bacterial spot and Pseudomonas syringae pv. syringae, causal agent of bacterial canker.
A detached-leaf bioassay was used to evaluate peach regenerants derived from ‘Sunhigh’ (susceptible to leaf spot and bacterial canker) and ‘Redhaven’ (moderately resistant to leaf spot) embryo callus cultures.
Twenty-six clones derived from calli produced on two ‘Sunhigh’ embryos, #61 and #156, and 19 clones derived from calli produced on three ‘Redhaven’ embryos, #30, #46 and #122 were evaluated.
A significantly greater number of clones with levels of leaf spot resistance greater than that found in both ‘Sunhigh’ and ‘Redhaven’ were obtained from calli derived from embryos #156 and #122. Sixty-four percent of the #156-clones and 33% of the #122-clones were more spot resistant than ‘Sunhigh’ and 9% of the #156-clones and 33% of #122-clones were more resistant than ‘Redhaven’. Of the ‘Sunhigh’ and ‘Redhaven’ seedlings screened, 22% of the ‘Sunhigh’ seedlings were more leaf spot resistant than ‘Sunhigh’ and 3% more resistant than ‘Redhaven’. None of the ‘Redhaven’ seedlings was more résistant than ‘Redhaven’.
Nine of the #156-clones and also plants propagated from these clones were evaluated for resistance to P. s. pv. syringae. Five of the original 9 clones were more canker resistant than ‘Sunhigh’. Of the clones produced through micropropagation, one continued to test more resistant than ‘Sunhigh’.
These studies suggest that the degree of somaclonal variation for leaf spot resistance is genetically determined and that screening for somaclonal variation may be a viable approach to obtaining peach plants with leaf spot and canker resistance.
Authors
F.A. Hammerschlag, V. Ognjanov
Keywords
Online Articles (95)