Home > I International Symposium on In Vitro Culture and Horticultural Breeding > IN VITRO FLOWER DEVELOPMENT OF THE NORMAL AND A MALE STERILE, STAMENLESS-2 MUTANT OF TOMATO (LYCOPERSICON ESCULENTUM MILL.)

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IN VITRO FLOWER DEVELOPMENT OF THE NORMAL AND A MALE STERILE, STAMENLESS-2 MUTANT OF TOMATO (LYCOPERSICON ESCULENTUM MILL.)

Article number

280_92

Pages

563 – 568

Language

Abstract

Young floral buds of the normal and male sterile stamenless-2 (sl-2/ sl-2) mutant of tomato were cultured successfully in vitro in modified Murashige and Skoog medium.
The normal buds required benzylaminopurine (BAP), whereas the mutant buds also needed gibberellic acid (GA₃) for growth and development.
Naphthaleneacetic acid (NAA) was inhibitory to the growth of all floral organs, and abscisic acid (ABA) and 2-chloroethyltrimethyl ammonium chloride (CCC) specifically inhibited the growth of stamens of normal floral buds.
The mutant buds grew to maturity in 4 to 5% sucrose; concentrations which were inhibitory to the normal buds.
The observations reported suggest that the sl-2/sl-2 mutation affects the endogenous gibberellins which through lower levels of soluble sugars, affect the development of pollen grains.

Publication

I International Symposium on In Vitro Culture and Horticultural Breeding

Authors

V.K. Sawhney, R. Rastogi

Keywords

Full text

https://doi.org/10.17660/ActaHortic.1990.280.92

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