Articles
SELECTION FOR SALT TOLERANCE IN REGENERATING CULTURES OF COLEUS BLUMEI
Article number
280_68
Pages
425 – 430
Language
Abstract
The purpose of this project was to compare the level of salt tolerance in Coleus blumei cv.
Xenia Field produced either from an in vitro selection or from an in vivo selection method.
In the in vitro selection, differentiated cultures, placed directly on a MS medium containing 90 mM NaCl, produced plantlets and finally plants.
A rooting test in NaCl concentrations up to 50 mM on cuttings derived from these plants showed enhanced tolerance, expressed as improved rooting performance.
In the in vivo selection a large number of C. blumei seedlings was screened in the presence of 90 mM NaCl, then the survivors grown to maturity.
The ability of cuttings of these plants to form roots in NaCl concentrations up to 50 mM was also assessed.
The in vitro derived cuttings showed better rooting performance than the seedling derived material.
Xenia Field produced either from an in vitro selection or from an in vivo selection method.
In the in vitro selection, differentiated cultures, placed directly on a MS medium containing 90 mM NaCl, produced plantlets and finally plants.
A rooting test in NaCl concentrations up to 50 mM on cuttings derived from these plants showed enhanced tolerance, expressed as improved rooting performance.
In the in vivo selection a large number of C. blumei seedlings was screened in the presence of 90 mM NaCl, then the survivors grown to maturity.
The ability of cuttings of these plants to form roots in NaCl concentrations up to 50 mM was also assessed.
The in vitro derived cuttings showed better rooting performance than the seedling derived material.
Authors
K.M. Ibrahim, H.A. Collin, J.C. Collins
Keywords
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