Articles
LONG-TERM SOMATIC EMBRYO PRODUCTION AND PLANT REGENERATION FROM EMBRYO-DERIVED PEACH CALLUS
Article number
280_32
Pages
183 – 190
Language
Abstract
Habituated embryogenic peach callus cultures have continuously produced somatic embryos and shoot-like structures for over 4 years.
Abscisic acid (ABA) at 5 μ M increased embryo production but did not improve shoot production.
After 18 months of culture on ½ strength Murashige and Skoog medium without growth regulators, 0.1 μM indolebutyric acid with 5 μ M benzyladenine induced 10% of the calli to form shoots.
After 4 years of culture shoot production was sporadic.
Embryogenic callus stored for up to 55 days at 4°C retained its embryogenic capacity.
Non-embryogenic sectors of callus were found to have lower levels of ABA, dihydrozeatin riboside and trans-zeatin riboside than embryogenic sectors.
Growth of embryogenic cultures was improved by the use of Driver-Kuniyuki-Walnut medium in place of Murashige and Skoog medium and by the use of Phytagel (an agar substitute) in place of TC Agar.
Abscisic acid (ABA) at 5 μ M increased embryo production but did not improve shoot production.
After 18 months of culture on ½ strength Murashige and Skoog medium without growth regulators, 0.1 μM indolebutyric acid with 5 μ M benzyladenine induced 10% of the calli to form shoots.
After 4 years of culture shoot production was sporadic.
Embryogenic callus stored for up to 55 days at 4°C retained its embryogenic capacity.
Non-embryogenic sectors of callus were found to have lower levels of ABA, dihydrozeatin riboside and trans-zeatin riboside than embryogenic sectors.
Growth of embryogenic cultures was improved by the use of Driver-Kuniyuki-Walnut medium in place of Murashige and Skoog medium and by the use of Phytagel (an agar substitute) in place of TC Agar.
Authors
R. Scorza, J.M. Cordts, S. Mante
Keywords
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