MEIOSIS AND FERTILITY IN ANTHER-CULTURE-DERIVED CABBAGE 2X-PLANTS

Of 36 anther-culture-derived 2x-plants of cabbage (Brassica oleracea L. ssp. capitata L.), 13 (36%) were male sterile (ACMS) and 23 (64%) were fertile (ACMF). Chi-square test was used to compare the percentage of pollen mother cells (PMCs) with meiotic abnormalities in ACMF or ACMS plants with those in the anther donor (control) plants.
In M-I, univalents, trivalents and quadrivalents were frequently observed but only in the ACMS group were these abnormalities significantly higher than in the control.
In A-I to T-I, there were significantly higher occurences of laggards and other abnormalities in both ACMF and ACMS groups.
In A-II to T-II, micronuclei, tripoles, unequal chromosome distribution and sporads with dyads and triads were frequently observed in both ACMF and ACMS but, in all cases, occurences were markedly higher in the ACMS group accompanied by significantly lower 9–9–9–9 equal chromosome segregation.
High incidence of meiotic abnormalities and sterility and low fertility in anther-culture-derived 2x-plants, particularly in the ACMS group, appears to be the result of homozygosity induced by anther culture.

I International Symposium on In Vitro Culture and Horticultural Breeding

M.S. Chiang, R. Crête, S. Fréchette, C. Chong

https://doi.org/10.17660/ActaHortic.1990.280.54