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Articles

AGROBACTERIUM-MEDIATED TRANSFORMATION OF APPLE AND STRAWBERRY USING DISARMED TI-BINARY VECTORS

Article number

280_82

Pages

495 – 502

Language

Abstract

The disarmed Ti-binary vector pBIN6 in Agrobacterium tumefaciens has been used to produce transgenic apple and strawberry plants from transformed leaf discs and petioles.
Genes encoding nopaline synthase (nos) and neomycin phosphotransferase (npt II) have been shown to be present in host DNA by Southern blot analyses.
Their expression has been confirmed by enzyme assay and rooting bioassays in the presence of kanamycin.
In the case of strawberry it was possible to perform inheritance studies on the segregation of nos by self-pollinating greenhouse grown plants.
The nos gene segregated 3:1 in in vitro germinated seedlings according to the expected Mendelian ratio for a single dominant.

Publication

I International Symposium on In Vitro Culture and Horticultural Breeding

Authors

D.J. James, A.J. Passey, D.J. Barbara

Keywords

Full text

https://doi.org/10.17660/ActaHortic.1990.280.82

Online Articles (95)

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V. Nuti Ronchi

3. TISSUE CULTURE AND THE GENETIC IMPROVEMENT OF GRAPEVINES: A REVIEW

M. G. Mullins

4. SYNTHETIC SEED TECHNOLOGY: IMPROVING DESICCATION TOLERANCE OF SOMATIC EMBRYOS OF CELERY

Y. Kim | J. Janick

5. BASIC PEROXIDASES AND ROOTING IN MICROCUTTINGS OF MALUS

G. Klerk | J. Brugge | R. Smulders | M. Benschop

6. LEAF-CALLUS GROWTH, SHOOT REGENERATION AND SOMACLONAL VARIATION IN ACTINIDIA DELICIOSA : EFFECT OF MEDIA PH

G. Marino | S. Battistini

7. THE USE OF A BRIEF 2,4-D TREATMENT TO INDUCE LEAF REGENERATION ON PRUNUS CANESCENS BOIS

M. Antonelli | Ph. Druart

8. IMPROVING YIELD OF ADVENTITIOUS SHOOTS IN APPLE

M. Dufour

9. CULTIVAR DEPENDENT RESPONSES TO REGENERATION FROM LEAVES IN APPLE

F. Fasolo Fabbri Malavasi | S. Predieri

10. FACTORS INFLUENCING SOMATIC EMBRYOGENESIS AND PLANTLET REGENERATION IN PECAN, CARYA ILLINOENSIS

Hazel Y. Wetzstein | James R. Ault | Scott A. Merkle

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B.L. Nadel | A. Altman | M. Ziv

12. ORGANOGENESIS AND EMBRYOGENESIS IN LISIANTHUS RUSSELLIANUS HOOK

B. Ruffoni | C. Damiano | F. Massabò | P. Esposito

13. ABSCISIC ACID INDUCED HIGH FREQUENCY EMBRYOGENESIS FROM SAPINDUS TRIFOLIATUS LEAVES

S.K. Unnikrishnan | A.R. Mehta | P.N. Bhatt

14. ADVENTITIOUS SHOOT FORMATION FROM IN VITRO LEAVES OF MM.106 APPLE CLONAL ROOTSTOCK

M. ANCHERANI | P. ROSATI | S. PREDIERI

15. TISSUE CULTURES OF BEAN (P. COCCINEUS L.) AND THEIR APPLICATIONS TO BREEDING

R. Angelini Rota | A. Genga | A. Allavena

16. IN VITRO SHOOT REGENERATION IN PASSIFLORA COERULEA

F. Vestri | S. Schiff | A. Bennici

17. ESTABLISHMENT OF AN IN VITRO RIBES GERMPLASM COLLECTION AND PRELIMINARY INVESTIGATIONS INTO LONG-TERM LOW TEMPERATURE GERMPLASM STORAGE

R.M. Brennan | S. Millam | D. Davidson | A. Wilshin

18. INDUCTION OF SOMATIC EMBRYOGENESIS IN GENISTA MONOSPERMA LAM.

P. Curir | B. Ruffoni | F. Massabò | C. Damiano

19. EFFECT OF CULTURE CONDITIONS AND LEAF SELECTION ON ORGANOGENESIS OF MALUS DOMESTICA CV. MCINTOSH “WIJCIK” AND PRUNUS CANESCENS BOIS GM79.

Ph. Druart

20. IMPROVEMENT OF SOMATIC EMBRYOGENESIS OF THE CHERRY DWARF ROOTSTOCK INMIL/GM9 BY THE USE OF DIFFERENT CARBON SOURCES

Ph. Druart

21. COMPARATIVE STUDIES ON IN VITRO REGENERATION OF SEEDLING EXPLANTS IN CHILI PEPPER (CAPSICUM ANNUUM L.)

M. Fári | Z. Túry | Mrs. F. Csillag

22. REGENERATION FROM ALSTROEMERIA CALLUS

E. Gonzalez Benito | P.G. Alderson

23. CALLUS FORMATION FROM TOMATO ENDOSPERM

V. Kagan-Zur | D. Mills | Y. Mizrahi

24. PROPAGATION AND ORGANOGENESIS “IN VITRO” OF ANACARDIUM OCCIDENTALE L.

A.R. Leva | A.M. Falcone

25. IN VITRO CLONAL PROPAGATION OF STRAWBERRY FROM IMMATURE ACHENES

E.K. Lis

26. ETHYLENE INVOLVEMENT IN IN VITRO REGENERATION OF LILY

A. Mensuali Sodi | M. Panizza | F. Tognoni

27. EFFECT OF LIGHT QUALITY ON REGENERATION FROM CALLUS OF ACTINIDIA DELICIOSA

R. Muleo | S. Morini

28. SOMATIC EMBRYOGENESIS IN CHESTNUT

C. Piagnani | T. Eccher

29. IN VITRO CULTURE TECHNIQUES FOR CARNATION BREEDING

L. Radojevic | N. Djordjevic | J. Petrovic

30. REGULATION OF ORGANOGENESIS WITH JASMONIC ACID

M. Ravnikar | J. Rode | N. Gogala | D. Benedicic

31. ADVENTIVE REGENERATION IN CELL COLONIES OF CARNATION

B. Ruffoni | F. Massabò | P. Curir | C. Guda

32. FIELD EVALUATION OF ANDROGENETIC LINES OF EGGPLANT

M.C. Sanguineti | R. Tuberosa | S. Conti

33. LONG-TERM SOMATIC EMBRYO PRODUCTION AND PLANT REGENERATION FROM EMBRYO-DERIVED PEACH CALLUS

R. Scorza | J.M. Cordts | S. Mante

34. EFFECT OF CYTOKININS ON EMBRYOGENIC CALLUS FORMATION FROM UNDEVELOPED OVULES OF ORANGE

A. Starrantino | P. Caponnetto

35. INFLUENCE OF TDZ AND BA ON ADVENTITIOUS SHOOT REGENERATION FROM APPLE LEAVES

C. Theiler-Hedtrich | R. Theiler-Hedtrich

36. ORGANOGENESIS AND SOMATIC EMBRYOGENESIS FROM CALLUS OF WINGED BEAN (PSOPHOCARPUS TETRAGONOLOBUS (L.) DC.)

S. Venketeswaran | M.A.D.L. Dias | Ursula V. Weyers

37. ORGANOGENESIS AND SOMATIC EMBRYOGENESIS FROM CALLUS OF WINGED BEAN (PSOPHOCARPUS TETRAGONOLOBUS (L.) DC.)

S. Venketeswaran | M.A.D.L. Dias | Ursula V. Weyers

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M. Ziv

39. PROTOPLAST TECHNOLOGY AND TOP-FRUIT TREE BREEDING

Sergio J. Ochatt

40. CELL ENGINEERING BY PROTOPLAST FUSION: IMPLICATIONS FOR PLANT IMPROVEMENT

A. Zelcer | S. Izhar | D. Wolf | B. Levy | O. Soferman

41. NON-AXENIC PLANT TISSUE CULTURE: POSSIBILITIES AND OPPORTUNITIES

Edwin B. Herman

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A. Vardi | A. Frydman-Shani | E. Galun | P. Gonen | S. Bliechman

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M. De Donato | C. Mozzetti | E. Perucco

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G. Marino

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Estela M. Patat-Ochatt | John B. Power

51. INFLUENCE OF LIGHT INTENSITY ON TOMATO CALLUS PRODUCTION

A.C. Wetten | I.P. Cumbus

52. INDUCTION OF HAPLOIDY IN FRUIT TREES

Y.X. Zhang | Y. Lespinasse | E. Chevreau

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G. Geraci | A. Starrantino

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M.S. Chiang | R. Crête | S. Fréchette | C. Chong

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M.P. Fuller | S. Turton | B.W.W. Grout

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M. Hoefer | V. Hanke

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A. Tosca | M. Lombardi | L. Marinoni | L. Conti | P. Frangi

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R. Theiler-Hedtrich

63. INHERITANCE AND STABILITY OF SOMACLONAL VARIATIONS IN MICROPROPAGATED STRAWBERRY

S. Sansavini | P. Rosati | D. Gaggioli | M.F. Toschi

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U. Laneri | R. Franconi | P. Altavista

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K.M. Ibrahim | H.A. Collin | J.C. Collins

70. IN VITRO SELECTION FOR SALT TOLERANCE IN COLEUS BLUMEI

J.C. Collins | K.M. Ibrahim | H.A. Collin

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P. Crino | A. Lai | R. Di Bonito | F. Saccardo

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M. De Donato | C. Mozzetti | E. Perucco

73. ETHYLENE PRODUCTION IN TISSUE CULTURE OF PEACH X ALMOND HYBRID, TOMATO, SWEET CHERRY, AND GRAPE

R. Jona | I. Gribaudo

74. A SOMACLONAL VARIANT IN CYMBIDIUM

U. Laneri

75. IN VITRO CULTURE OF CUCUMIS SATIVUS XI. SELECTION OF RESISTANCE TO FUSARIUM OXYSPORUM

S. MALEPSZY | A. EL-KAZZAZ

76. IN VITRO GROWTH PATTERN OF SALT-STRESSED CELLS OF LAVANDIN

A. Mensuali Sodi | G. Serra | C. Vitagliano | F. Blando

77. IN VITRO PROPAGATION OF LAVANDIN: MORPHOLOGICAL CHANGES IN REGENERATED PLANTS

M. Panizza | F. Tognoni | A. Mensuali Sodi

78. EFFECT OF PHOMA TRACHEIPHILA TOXINS ON CITRUS PROTOPLASTS

F. Sesto | V. Grimaldi | A.M. Pennisi

79. INDUCTION AND USE OF VARIATION IN POTATO WITH DIFFERENT IN VITRO SYSTEMS

80. PRODUCTION AND STUDIES OF FERTILE INTERGENERIC HYBRIDS LYCOPERSICON ESCULENTUM MILL. X SOLANUM LYCOPERSICOIDES DUN

P.K. Stoeva | Z.V. Achkova | N.P. Zagorska

81. ION LEAKAGE IN THE PRESENCE OF FUNGAL TOXINS AS A METHOD FOR SCREENING RESISTANT GENOTYPES IN TOMATO AND CARNATION

82. FACTORS INFLUENCING VIRULENCE IN AGROBACTERIUM-MEDIATED TRANSFORMATION OF APPLE

A.M. Dandekar | S.L. Uratsu | N. Matsuta

83. AGROBACTERIUM-MEDIATED TRANSFORMATION OF APPLE AND STRAWBERRY USING DISARMED TI-BINARY VECTORS

D.J. James | A.J. Passey | D.J. Barbara

84. THE POTENTIAL FOR GENETIC MANIPULATION OF PEACH FRUIT DEVELOPMENT AND QUALITY THROUGH DIRECTED GENE TRANSFER

85. LIPOSOME-MEDIATED INTRODUCTION OF DNA INTO PLANT PROTOPLASTS AND CALLI

86. PLANTLET REGENERATION AND GENETIC TRANSFORMATION IN SOFT FRUIT SPECIES

J. Graham | Ronald J. McNicol

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E. Filippone | Paul F. Lurquin

88. TOWARDS GENETIC TRANSFORMATION OF BEAN BY AGROBACTERIUM TUMEFACIENS.

A. Genga | A. Allavena | A. Ceriotti | R. Bollini

89. RESISTANCE TO BACTERIAL DISEASE: PRELIMINARY RESULTS OF A BIOTECHNOLOGICAL APPROACH IN POTATO

C. MONTANELLI | G. NASCARI

90. THE POTENTIAL USE OF RESTRICTION FRAGMENT LENGTH POLYMORPHISM IN RUBUS BREEDING

R. Waugh | M. van de Van | S. Millam | R. Brennan | W. Powell

91. MOLECULAR PHENOGENETICS AS AN AID TO FRUIT BREEDING

Don J. Durzan

92. ISOLATION, CHARACTERIZATION AND EXPRESSION OF TOMATO SUPEROXIDE DISMUTASE GENES – AN UPDATE AND COMPARISON WITH SIMILAR GENES FROM OTHER PLANTS

E. Galun | R. Perl-Treves | B. Nacmias | N. Magal | D. Aviv

93. IN VITRO FLOWER DEVELOPMENT OF THE NORMAL AND A MALE STERILE, STAMENLESS-2 MUTANT OF TOMATO (LYCOPERSICON ESCULENTUM MILL.)

V.K. Sawhney | R. Rastogi

94. GENE EXPRESSION AND EPIPHYLLOUS BUD REACTIVATION IN KALANCHOE MORTAGEI

Y.T. JASRAI | S. UNNIKRISHNAN | P.N. BHATT | A.R. MEHTA

95. EXPRESSION OF THE PLUM POX VIRUS COAT PROTEIN GENE IN NICOTIANA CLEVELANDII