FLOW CYTOMETRIC ASSESSMENT OF VIABILITY AND DIFFERENTIATION IN POTATO AND ORANGE CELL SUSPENSION PROTOPLASTS.

Flow cytometry (FCM), a relatively novel technique in plant cell studies, is based on the analysis of the fluorescent emissions from single cells as they flow one-by-one past a fluorescence detector.
It has proved most effective in detecting the first signs of a decay in viability or the commencement of differentiation in protoplast cultures.
We have applied FCM to potato (Solanum tuberosum) and orange (Citrus sinensis) in order to check the ploidy level of our explants, the viability of isolated protoplasts and their ability to regenerate cell walls.

FCM proved to be an accurate, fast and effective technique to analyse isolated nuclei and whole protoplasts following staining by different fluorochromes: 4′,6-diamidino-2-phenylindole (DAPI) was used for extracted nuclei (Sgorbati et al., 1986); fluorescein diacetate (FDA) and Calcofluor White (CFW) were utilized for labelling protoplasts (Berglund et al., 1988; Galbraith, 1981).

I International Symposium on In Vitro Culture and Horticultural Breeding

S. Lucretti, A. Lister, J.V. Chapman, F. Moretti, F. De Giacometti, P. Ianni Palarchio

https://doi.org/10.17660/ActaHortic.1990.280.47