INFLUENCE OF SONICATION ON REGENERATION AND TRANSFORMATION EFFICIENCIES IN APPLE

Based on the observation that most of the shoot regeneration from apple leaf explants is associated with cutting edges and wounds caused by forceps and that Agrobacterium tumefaciens-mediated transformation depends on wounding (Norelli et al., 1996) we tested the influence of sonication on regeneration and transformation efficiencies by using the SAAT-method (Sonication Assisted Agrobacterium-mediated Transfer, Trick and Finer, 1997). Leaf explants from three apple varieties, Holsteiner Cox, Gloster and Elstar, were treated in Agrobacterium (EHA101, pIBGUS) suspension with sonication for various timed intervals (10, 20, 40 and 80s). First results indicate an increased stable -glucuronidase expression in ‘Holsteiner Cox’ leaf tissue.
Improvement of transformation efficiencies seems to be genotype dependent, because the transformation efficiencies in ‘Elstar’ and ‘Gloster’ were not influenced.
Regeneration rates of the three varieties, however, are drastically reduced through sonication, depending on the duration of the treatment.

IV International Symposium on In Vitro Culture and Horticultural Breeding

I. Szankowski, A. Lübke, H.-J. Jacobsen

apple, Malus, sonication, transformation, regeneration

https://doi.org/10.17660/ActaHortic.2001.560.102