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Articles

IN VITRO PROPAGATION OF DWARFED ROOTSTOCKS FOR SWEET CHERRY

Article number

560_95

Pages

477 – 480

Language

English

Abstract

Dwarfed rootstocks for sweet cherry were bred in RIBP Holovousy.
The results obtained during 25-year observation have shown the growth reduction of grafted cultivars.
This growth reduction reached 70% for P-HL-A, 50% for P-HL-B, and 80% for P-HL-C in comparison with seedling, depending on the cultivar.
However, these cultivars are extremely difficult to direct rooting.
That is why the sweet cherry rootstocks P-HL have been propagated commercially through in vitro culture.
We have developed the method for multipropagation on MS (Murashige-Skoog) medium with different BAP (6-benzylaminopurine) concentrations.
The optimum concentration for multiplication was 1.5 mg BAP in 1 litre of MS medium.
Multiplication rate was by 50% higher than the control variant (0.75 mg 1-1 BAP). The addition of phloroglucinol in rooting phase had stimulated effect on the shoot growth.
Rooting and transfer to ex vitro condition are under investigation.

Publication

IV International Symposium on In Vitro Culture and Horticultural Breeding

Authors

M. Erbenová, F. Paprstein, J. Sedlák

Keywords

sweet cherry, dwarfed rootstocks, propagation, in vitro, 6-benzylaminopurine

Full text

https://doi.org/10.17660/ActaHortic.2001.560.95

Online Articles (117)

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J. Sedlák | F. Paprstein | A. Bilavcík | J. Zámecník

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Parveen | E.H. Birnbaum

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A.E. Ramirez | E.H. Birnbaum

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T. Shimada | M. Otani | M. Morii

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