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Articles

ESTABLISHMENT OF A SEMICONTINUOUS BIOREACTOR CULTURE OF PHYSCOMITRELLA PATENS FOR MASS PRODUCTION OF PROTOPLASTS

Article number

560_82

Pages

425 – 428

Language

English

Abstract

For large scale protoplast transformation of the moss Physcomitrella patens (Hedw.) B.S.G. semicontinuous protonema suspension cultures in bioreactors were established and optimised with regard to the yield of protoplasts.
Supplementation of Knop medium with 2.5 mM ammonium tartrate markedly improved the protoplast yield (7.4 x 10⁴ protoplasts/mg dry weight). Bioreactor culture was performed in 5 l vessels.
By harvesting on average 1100 ml/day which corresponds to a dilution rate of 0.22/d a semicontinuous culture was maintained for 49 days yielding 51 l of suspension culture.
Beginning eleven days after the start of the bioreactor run around 6 % of the cells were polyploid (4C) which might indicate aging of the culture caused by phytohormone accumulation.

Publication

IV International Symposium on In Vitro Culture and Horticultural Breeding

Authors

A. Hohe, G. Schween, R. Reski

Keywords

ammonium tartrate, flowcytometric analysis, moss, suspension culture

Full text

https://doi.org/10.17660/ActaHortic.2001.560.82

Online Articles (117)

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