Home > IV International Symposium on In Vitro Culture and Horticultural Breeding > INCREASED RESISTANCE TO ERWINIA AMYLOVORA EXHIBITED BY TRANSGENIC ‘ROYAL GALA’ APPLE (MALUS x DOMESTICA BORKH.) SHOOTS, CARRYING A MODIFIED CECROPIN MB39 GENE

Articles

INCREASED RESISTANCE TO ERWINIA AMYLOVORA EXHIBITED BY TRANSGENIC ‘ROYAL GALA’ APPLE (MALUS x DOMESTICA BORKH.) SHOOTS, CARRYING A MODIFIED CECROPIN MB39 GENE

Article number

560_11

Pages

95 – 99

Language

English

Abstract

Transgenic ‘Royal Gala’ apple (Malus x domestica Borkh.) shoots were obtained by Agrobacterium-mediated gene transfer using a vector with T-DNA encoding a modified cecropin gene MB39 joined to a secretory coding sequence from barley α-amylase, and placed under the control of a wound-inducible tobacco osmotin promoter.
The integration of the cecropin MB39 gene into apple was confirmed by Southern blot analysis.
Three of the seven transgenics exhibited 2.5- to 3.3-fold more resistant to Erwinia amylovora than the non-transformed ‘Royal Gala’ control, demonstrating the potential of introducing the cecropin gene to increased levels of disease resistance.

Publication

IV International Symposium on In Vitro Culture and Horticultural Breeding

Authors

Q. Liu, J. Ingersoll, L. Owens, S. Salih, R. Meng, F. Hammerschlag

Keywords

Agrobacterium tumefaciens, fire blight, gene transfer

Full text

https://doi.org/10.17660/ActaHortic.2001.560.11

Online Articles (117)

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12. INCREASED RESISTANCE TO ERWINIA AMYLOVORA EXHIBITED BY TRANSGENIC ‘ROYAL GALA’ APPLE (MALUS x DOMESTICA BORKH.) SHOOTS, CARRYING A MODIFIED CECROPIN MB39 GENE

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P.A. Sansberro | H.Y. Rey | C.V. Luna | L.A. Mroginski

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J. Sedlák | F. Paprstein | A. Bilavcík | J. Zámecník

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A.E. Ramirez | E.H. Birnbaum

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D. Rzepka-Plevnes | J. Kurek

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M. Erbenová | F. Paprstein | J. Sedlák

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T. Shimada | M. Otani | M. Morii

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Jiu-Sherng Yang

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