Home > IV International Symposium on In Vitro Culture and Horticultural Breeding > A COMPARISON OF DIRECT AND INDIRECT SOMATIC MORPHOGENESIS FOR THE PRODUCTION OF TRANSGENIC SUGARCANE (SACCHARUM SPP. HYBRIDS)

Articles

A COMPARISON OF DIRECT AND INDIRECT SOMATIC MORPHOGENESIS FOR THE PRODUCTION OF TRANSGENIC SUGARCANE (SACCHARUM SPP. HYBRIDS)

Article number

560_13

Pages

105 – 108

Language

English

Abstract

The conventional genetic transformation protocol for sugarcane employs microprojectile bombardment of embryogenic callus.
We report an approach involving bombardment of leaf explants, followed by direct somatic embryogenesis.
The technique involves gene delivery to immature leaf roll segments cultured for only 2 weeks on MS with 0.3 mg 1^-1 2,4-D. When this method was compared to the conventional one by producing transgenic plants containing the pat gene, PCR-determined transformation efficiencies were 72 and 100%, respectively.
However, the newly-developed protocol reduced plantlet production time and number of subcultures, thereby reducing the cost of transgenic plant production from US$ 33 to US$ 2.7 per plant.

Publication

IV International Symposium on In Vitro Culture and Horticultural Breeding

Authors

S.J. Snyman, B.I. Huckett, M.P. Watt, F.C. Botha

Keywords

2,4-D concentration, production costs, target material, somatic embryogenesis

Full text

https://doi.org/10.17660/ActaHortic.2001.560.13

Online Articles (117)

2. WORDS OF GREETINGS FROM THE MINISTRY OF AGRICULTURE AND FORESTRY OF FINLAND TO THE SYMPOSIUM

L. Hömmö

3. GENETIC ENGINEERING FOR YELLOW FLOWER COLOURS

4. GENETIC ENGINEERING FOR DISEASE RESISTANCE: ANTIFUNGAL PROTEINS/PEPTIDES TO THE RESCUE

K. Rajasekaran | J.W. Cary | T.J. Jacks | T.E. Cleveland

5. CRYPTIC GENETIC ELEMENTS FOR REGULATION OF GENE EXPRESSION IN PLANTS: THE tCUP GENE EXPRESSION SYSTEM

6. USING HETEROLOGOUS AND HOMOLOGOUS PROMOTERS TO STUDY TISSUE SPECIFIC TRANSGENE EXPRESSION IN FRUIT CROPS

7. DNA MARKERS FOR DIFFERENT ASPECTS OF PLANT BREEDING RESEARCH AND ITS APPLICATIONS

H. Nybom

8. MEDIUM- AND LONG-TERM CONSERVATION AND SAFE INTERNATIONAL EXCHANGE OF GERMPLASM FROM FOOD AND CASH TROPICAL CROPS

B. Malaurie

9. CRYOPRESERVATION OF PLANT GERMPLASM

B. Panis | R. Swennen | F. Engelmann

10. MANIPULATING THE GIBBERELLIN RESPONSE TO REDUCE PLANT HEIGHT IN CHRYSANTHEMUM MORIFOLIUM

L.M. Petty | B. Thomas | S.D. Jackson | N. Harberd

11. GENETIC ENGINEERING OF AGRONOMIC AND ORNAMENTAL TRAITS IN CARNATION

12. INCREASED RESISTANCE TO ERWINIA AMYLOVORA EXHIBITED BY TRANSGENIC ‘ROYAL GALA’ APPLE (MALUS x DOMESTICA BORKH.) SHOOTS, CARRYING A MODIFIED CECROPIN MB39 GENE

13. STABLE TRANSFORMATION OF PIONSETTIA VIA ELECTROPHORESIS

N.I. Vik | A.K. Hvoslef-Eide | H. Gjerde | K. Bakke

14. A COMPARISON OF DIRECT AND INDIRECT SOMATIC MORPHOGENESIS FOR THE PRODUCTION OF TRANSGENIC SUGARCANE (SACCHARUM SPP. HYBRIDS)

S.J. Snyman | B.I. Huckett | M.P. Watt | F.C. Botha

15. INDUCTION OF TETRAPLOIDS FOR BREEDING HARDY ORNAMENTALS

J.B. Rose | J. Kubba | K.R. Tobutt

16. INTRODUCTION OF THE ATMOSPHERIC NITROGEN FIXING ABILITY TO PLANTS: DIRECT BACTERIUM BOMBARDMENT

E. Preininger | K. Boka | P. Korányi | I. Gyurján | J. Zatykó

17. CRYOPRESERVATION TRIALS IN CARICA PAPAYA

S.E. Ashmore | M. Azimi | R.A. Drew

18. CRYOPRESERVATION OF FOREST TREE GERMPLASM

H. Häggman | L. Ryynänen | T. Aronen

19. CRYOPRESERVATION OF WOODY SPECIES BY VITRIFICATION OF SHOOT TIPS AND EMBRYOGENIC TISSUE

M. Lambardi | A. De Carlo | C. Benelli | G. Bartolini

20. TRANSFORMATION OF BEAN CALLUS BY USING HIGH-VELOCITY MICROPROJECTILES

E. Eissa Ahmed | GY. Bisztray | I. Velich

21. CHARACTERISATION ON OKRA (Hibiscus esculentus L.) GERM-PLASM IN TURKEY

T. Bas | J. Koludar

22. VITRIFICATION OF SHOOT LIPS, NODAL SEGMENTS AND EMBRYOGENIC TISSUE OF OLIVE (olea europaea L.) FOR GERMPLASM CRYOPRESERVATION

C. Benelli | A. De Carlo | M. Lambardi | P.T. Lynch

23. CARNATION (Dianthus caryophyllus L.) DIHAPLOID LINES RESISTANT TO FUSARIUM OXYSPORUM F.SP. DIANTHI

R. Dolcet-Sanjuan | E. Clavería | Llauradó | A. Ortigosa | P. Arús

24. FLOWER DEVELOPMENT IN GERBERA HYBRIDA (ASTERACEAE)

25. GENETIC VARIABILITY OF ANTHER DONOR VERSUS SPONTANEOUS DOUBLED HAPLOID DESCENDENTS AND COLCHICINE INDUCED DOUBLED HAPLOID SWEET PEPPER (Capsicum annuum L.) LINES

26. IN VITRO GERMPLASM PRESERVATION OF LILY SPECIES UTILIZING CALLUS CULTURES AT LOW TEMPERATURE

Toshinari Godo | Masahiro Mii

27. FIRST RESULTS OF PELARGONIUM SHOOT APEX CRYOPRESERVATION

A. Grapin | C. Hupel | C. Marche | N. Dorion

28. PROSPECTS OF IN VITRO METHODS IN THE BREEDING OF ROSA RUGOSA IN LATVIA

G. Jakobsone

29. EXPRESSION OF THE BAR AND UIDA GENES BY GLADIOLUS FOLLOWING THREE SEASONS OF DORMANCY

K. Kamo

30. OPTIMISING OF THE TRANSFORMATION METHODS OF BLACK CURRANT (Ribes nigrum L. ) AND DEVELOPMENT OF TRANSGENIC RESISTANCE AGAINST BLACK CURRANT REVERSION VIRUS

R. Karjalainen | K. Välimäki | C. Pacot-Hiriart | M. Kleemola | K. Lehto

31. VARIATION IN PROPORTIONS OF GLYCOALKALOID AGLYCONES IN SOMATIC HYBRIDES BETWEEN TO POTATO SPECIES (Solanum tuberosum and brevidenS) POSSESSING DIFFERENT GENOME CONSTITUTIONS

32. MOLECULAR ANALYSIS OF CYTOPLASMIC MALE STERILITY IN BEETS

T. Mikami | T. Kubo | M.P. Yamamoto | E. Hagihara

33. AGROBACTERIUM-MEDIATED TRANSIENT EXPRESSION OF β-GLUCURONIDASE AND LUCIFERASE GENES IN LIMONIUM SINUATUM

M. Kimizou | Y. Yamamoto | T. Iwasaki | S. Ohki

34. INVESTIGATION ON MOLECULAR MARKERS FOR CHARACTERIZATION OF PARENTAL GENOTYPES USED IN RIBES SEXUAL HYBRIDIZATION

K. Wiejacha | S. Pluta | A. Golis | T. Orlikowska

35. FACTORS INFLUENCING LONG-TERM STORAGE OF STRAWBERRY SHOOTS IN VITRO

A. Lisek | T. Orlikowska

36. GENETIC TRANSFORMATION OF SWEET POTATO (Ipomoea batatas (L.) LAM.) BY AGROBACTERIUM TUMEFACIENS

M. Otani | Y. Wakita | T. Shimada

37. RELATIONSHIP BETWEEN PRODUCTION OF ALBINO BERRIES AND MIXOPLOIDY IN THE STRAWBERRY (Fragaria x ananassa) CV. KENT

S. Plante | H. Rousseau

38. REGULATION OF ANTHOCYANIN BIOSYNTHESIS IN ANTIRRHINUM

K. Schwinn | K. Davies | V. Alm | V. Mackay | C. Martin

39. TRANSGENIC PARTHENOCARPY IN DETERMINATE INDETERMINATE TOMATO CULTIVARS

R. Barg | S. Shabtai | N. Carmi | M. Pilowsky | Y. Salts

40. EFFECT OF AGROBACTERIUM INCUBATION CONDITIONS ON GENE TRANSFER AFFECIENCY ‘GALA’ AND ‘MCINTOSH WIJCIK’ APPLE

K.J. Song | S.W. Park | S.Y. Ahn | J.H. Hwang | Y.U. Shin

41. GENOTYPE EFFECTS ON AGROBACTERIUM MEDIATED GENETIC TRANSFORMATION OF BRASSICA NAPUS

K. Sonntag | B. Döscher | M. Sellner

42. SOMATIC HYBRIDISATION BETWEEN BRASSICA SPP. AND RAPHANUS SATIVUS

J. Müller | K. Sonntag | E. Rudloff

43. CRYOPRESERVATION OF IN VITRO SUGAR BEET (Beta vulgaris) SHOOT TIPS

B. Vandenbussche | M. De Proft

44. IN VITRO FORMATION OF ADVENTITIOUS MERISTEM AND ITS SIGNIFICANCE FOR MUTATION BREEDING OF DENDRANTHEMA GRANDIFLORA TZVELEV

M. Zalewska

45. WHY IS IT DIFFICULT TO OBTAIN TRANSGENIC PEPPER PLANTS?

D. Wolf | T. Matzevitch | B. Steinitz | A. Zelcer

46. SOMATIC EMBRYOGENESIS, PLANT REGENERATION AND GENETIC TRANSFORMATION IN FRAGARIA spp.

R. Donnoli | F. Sunseri | G. Martelli | I. Greco

47. DATE PALM (Phoenix dactylifera L. ) GERMPLASM BANK IN KING FAISAL UNIVERSITY, SAUDI ARABIA. SURVIVAL AND ADAPTABILITY OF TISSUE CULTURED PLANTLETS

A.S. Al-Ghamdi

48. ADVANCES AND CHALLENGES IN BIOREACTOR DESIGN FOR THE PRODUCTION OF CHEMICALS FROM PLANT TISSUE CULTURE

W.R. Curtis | E.B. Carvalho | L.D. Tescione

49. IDENTIFICATION OF SECONDARY METABOLITES IN IN VITRO CULTURE OF AMMI MAJUS TREATED WITH ELICITORS

A. Królicka | E. Lojkowska | I. Staniszewska | E. Malinski | J. Szafranek

50. EFFECTS OF NH₄⁺ : NO₃^– RATIO AND IONIC STRENGTH ON ADVENTITIOUS ROOT GROWTH AND GINSENOSIDE PRODUCTION IN BIOREACTOR CULTURE OF PANAX GINSENG C.A. MEYER

Kee-Won Yu | Eun-Joo Hahn | Kee-Yoeup Paek

51. APPLICATION OF PROTOPLAST FUSION TECHNOLOGY TO TANSY (Tanacetum vulgare L.) BIODIVERSITY AS A SOURCE TO ENHANCE BIOLOGICAL ACTIVITY OF SECONDARY COMPOUNDS

M.K. Keskitalo

52. EFFECT OF PPM ON ALKALOID PRODUCTION IN NARCISSUS CONFUSUS SHOOT-CLUMPS

R. Colque | F. Viladomat | J. Bastida | C. Codina

53. TWO BIOACTIVE COMPOUNDS AND A NOVEL CHALCONE SYNTHASELIKE ENZYME IDENTIFIED IN GERBERA HYBRIDA

S. Koskela | P. Elomaa | Y. Helariutta | P. Söderholm | P. Vuorela

54. COLD ACCLIMATION AND DEVELOPMENT OF FREEZING AND DROUGHT TOLERANCE IN PLANTS

55. BIOTECHNOLOGY OF PLANT OSMOTIC STRESS TOLERANCE PHYSIOLOGICAL AND MOLECULAR CONSIDERATIONS

W.X. Wang | B. Vinocur | O. Shoseyov | A. Altman

56. INDUCTION OF THE EMBRYOGENIC PATHWAY IN SOMATIC PLANT CELLS

A. Fehér | T. Pasternak | P. Miskolczi | F. Ayaydin | D. Dudits

57. ANCYMIDOL EFFECTS ON OXIDATIVE STRESS AND THE REGENERATION POTENTIAL OF NARCISSUS LEAVES IN LIQUID CULTURE

Jianxin Chen | Meira Ziv

58. SUCROSE ENHANCES COLD HARDENING OF RASPBERRY PLANTS IN VITRO

P. Palonen | O. Junttila

59. CHILL-INDUCED REACTIONS IN POPLAR

J. Renaut | J.F. Hausman | S. Lutts

60. SCREENING COLD HARDY STRAWBERRY SEEDLINGS UTILIZING IN VITRO TECHNOLOGY

V. Stanys

61. INDUCTION OF STRESS TOLERANCE IN POTATO MICROPLANTS BY HYDROGEN PEROXIDE IN VITRO

E. Rusite | D. Gertnere

62. POLYPLOIDISATION OF RHODODENDRON CULTIVARS IN VITRO AND HOW IT AFFECTS COLD HARDINESS

ANU Väinölä | TAPANI Repo

63. TRANSFORMATION OF APPLE FOR DURABLE SCAB RESISTANCE: EXPRESSION OF A PUROINDOLINE GENE IN SUSCEPTIBLE AND RESISTANT (Vf) GENOTYPES

E. Chevreau | F. Dupuis | C. Ortolan | L. Parisi

64. THE RELATIONSHIP BETWEEN THE ENDOGENOUS ABSCISIC ACID CONTENT OF ANTHERS AND IN VITRO ANDROGENESIS IN PEPPERS (Capsicum annuum L.)

D. Öskum | R. Tipirdamaz | S. Ellialtioglu

65. THE USE OF APOMIXIS IN CLONING HORTICULTURAL PLANTS: CURRENT APPLICATIONS AND MOLECULAR PROSPECTS

A.M. Koltunow | N.S. Scott | A.M. Chaudhury

66. BIOREACTOR TECHNOLOGY IN PLANT CLONING

D.J. Cantliffe

67. CONTAMINATION AND ITS IMPACT IN TISSUE CULTURE

A.C. Cassells

68. GENETIC HETEROGENEITY OF CLONES IN DIFFERENT TYPES OF APOMIXIS IN VITRO AND IN VIVO

T.B. Batygina

69. MOLECULAR TISSUE CULTURE PROCESSING (MTCP): A MOLECULAR AND PHYSIOLOGICAL METHOD TO OPTIMIZE IN VITRO PLANT REGENERATION SYSTEMS IN RECALCITRANT SPECIES

70. THE DISADVANTAGED EMBRYO

C.H. Bornman | O.S.P. Dickens | A.M. Botha

71. DEVELOPMENT OF NEW TISSUE CULTURE MEDIA, USING THE RELATION BETWEEN MINERAL COMPOSITION OF PLANT AND MEDIUM

H. Bouman | B. Morris | A. Tiekstra

72. PLANT PROPAGATION IN VITRO: HORMONAL INTERACTIONS

S.P.O. Werbrouck | H. Dhuyvetter | R.M. Pérez | N. Topoonyanont | P.C. Debergh

73. A LARGE SCALE PRODUCTION OF LILIUM BULBLETS THROUGH BIOREACTOR CULTURE

Y.S. Kim | E.J. Hahn | K.Y. Paek

74. MINERAL UPTAKE BY BANANA (Musa acuminata L.) EXPLANT IN VITRO

M.E. Amiri

75. SOMATIC EMBRYOGENESIS AND PLANT REGENERATION FROM OVARIES OF TULIPA GESNERIANA L. IN IN VITRO CULTURES

A. Bach | A. Ptak

76. IN VITRO CULTURE OF HUNGARIAN APRICOT (Prunus armeniaca L.) VARIETIES

I. Balla | J. Vértesy

77. EFFECTS OF AGAR IMPURITIES ON IN VITRO PROPAGATION OF RANUNCULUS ASIATICUS L.

M. Beruto | C. La Rosa | C. Portogallo

78. IN VITRO ADVENTITIOUS SHOOT REGENERATION FROM VEGETATIVE APICES OF ALMOND AND OTHER PRUNUS SPECIES

P. Lauri | E. Caboni | C. Damiano

79. EFFECTS OF ABA, FLURIDONE AND LIGHT QUALITY ON GROWTH AND DORMANCY OF TISSUE-CULTURED HERBACEOUS PEONY SHOOTS

E. Gabryszewska

80. CULTURE OF VERY YOUNG PHASEOLUS VULGARIS L. PODS AND PLANTLET REGENERATION

P. Geerts | A. Toussaint | G. Mergeai | J.P. Baudoin

81. CHANGES OF PHENOLIC COMPOUNDS OF STRAWBERRY PLANTLETS IN VITRO UNDER INDUCTIVE CONDITIONS

G. Günver

82. SHOOT REGENERATION FROM STEM AND LEAF EXPLANTS OF CHRYSANTHEMUM (Dendranthema x grandiflorum)

J.P. Himstedt | H.J. Jacobsen | Fischer-Klüver

83. ESTABLISHMENT OF A SEMICONTINUOUS BIOREACTOR CULTURE OF PHYSCOMITRELLA PATENS FOR MASS PRODUCTION OF PROTOPLASTS

A. Hohe | G. Schween | R. Reski

84. REGENERATIVE RESPONSES OF CYPHOMANDRA BETACEA (CAV.) SENDT. (TAMARILLO) CULTIVATED IN VITRO

M. Obando | M. Jordan

85. OPTIMIZING SHOOT PROLIFERATION AND ROOTING OF MICROPROPAGATED JAPANESE QUINCE (Chaenomeles japonica (Thunb.) Lindl. ex. Spach)

S. Kauppinen

86. IN VITRO STUDIES ON CALLUS INDUCTION IN BOTH VEGETATIVE AND GENERATIVE PARTS IN ALSTROEMERIA FOR FURTHER APPLICATION TO TRANSFORMATION

J.B. Kim | M. de Jeu | K.J.J.M. Raemakers | E. Jacobsen | R.G.F. Visser

87. THE INFLUENCE OF EXOGENOUS CYTOKININS ON THE PROLIFERATION OF EMBRYOGENIC TISSUE AND SOMATIC EMBRYO MATURATION OF NORWAY SPRUCE

M.J. Latkowska | H. Chmiel | K. Molska

88. MICROPROPAGATION IN VITRO AND REGENERATION OF FERTILE PLANTS OF THE ENDANGERED MEDICINAL SPECIES HARPAGOPHYTUM PROCUMBENS AND ZEYHERI

G. Levieille | G. Wilson

89. EFFECT OF LIGHT QUALITY ON IN VITRO PRODUCTION OF CALLUS IN EXPLANTS OF THREE POINSETTIA CULTIVARS

C. D'Onofrio | S. Morini

90. INFLUENCE OF GELLING AGENTS ON ILEX PARAGUARIENSIS TISSUE CULTURE

P.A. Sansberro | H.Y. Rey | C.V. Luna | L.A. Mroginski

91. ADAPTATION OF APPLE AND PEAR PLANTS TO IN VITRO CONDITIONS AND TO LOW TEMPERATURE

J. Sedlák | F. Paprstein | A. Bilavcík | J. Zámecník

92. IN VITRO PROPAGATION OF HALOXYLON APHYLLUM (MINKW.) ILJIN

Parveen | E.H. Birnbaum

93. INDUCTION AND CHARACTERIZATION OF CALLUS IN HALOXYLON APHYLLUM (MINKW.) ILJIN

A.E. Ramirez | E.H. Birnbaum

94. IN VITRO CULTURE OF PAPAVER NUDICAULE L.

M. Savona | L. Semeria | A. Allavena | S. Carli | P. Profumo

95. THE INFLUENCE OF MEDIA COMPOSITION ON THE PROLIFERATION AND MORPHOLOGY OF FICUS BENJAMINA PLANTLETS

D. Rzepka-Plevnes | J. Kurek

96. IN VITRO PROPAGATION OF DWARFED ROOTSTOCKS FOR SWEET CHERRY

M. Erbenová | F. Paprstein | J. Sedlák

97. IN VITRO PROPAGATION AND RECOVERY OF A HABITAT OF HABENARIA RADIATA (ORCHIDACEAE)

T. Shimada | M. Otani | M. Morii

98. ORGANOGENESIS AND ECOLOGICAL DIFFERENTIATION OF CYMBIDIUM SPECIES

K. Shimasaki | J. Tsuneishi | Y. Fukumoto

99. PAPAYA SOMATIC EMBRYO INDUCTION FROM FRUIT-BEARIN FIELD PLANTS: EFFECTS OF ROOT SUPPORTING MATERIAL AND POSITTION OF THE ROOT EXPLANTS

Jiu-Sherng Yang

100. THE EFFECT OF WOOD PROPERTIES UPON MICROPROPAGATION EFFICIENCY IN HYBRID ASPEN CLONES

101. THE ROLE OF ABA IN INDUCTION OF ANDROGENESIS IN BARLEY

S. van Bergen | R. van der Meulen | M. Wang

102. THE INFLUENCE OF GENOTYPE AND EXPLANT FOR CALLUS INDUCTION AND PROLIFERATION OF ROSA FLORIBUNDA

S. Kuusiene | M. Kandzezauskaite

103. INFLUENCE OF SONICATION ON REGENERATION AND TRANSFORMATION EFFICIENCIES IN APPLE

I. Szankowski | A. Lübke | H.-J. Jacobsen

104. SOMATIC EMBRYOGENESIS OF SILVER FIR (Abies alba Mill.) – POLISH PROVENANCES

K. Szczygiel | J. Kowalczyk

105. MICROWAVE STERILIZATION OF TISSUE CULTURE MEDIA

E.M.A. Youssef | G.A. Amin

106. CYCLIC SOMATIC EMBRYOGENESIS AND SCHEME FOR MULTIPLICATION OF QUERCUS ILEX L.

P.V. Mauri | J.A. Manzanera | M.M. Marcote

107. TRUE-TO TYPE PROTOCLONES REGENERATION FROM MESOPHYLL PROTOPLASTS OF “INMIL” CHERRY ROOTSTOCK (Prunus incisa x serrula)

V. Kondakova | PH. Druart

108. THE POTENTIAL FOR GENE FLOW FROM TRANSGENIC CROPS TO RELATED WILD SPECIES: A CASE STUDY IN STRAWBERRY

109. CHROMOSOME NUMBER DEPENDENT GENOME SIZE AND RAPD FINGERPRINTING DIAGNOSTICS FOR GENETIC INTEGRITY OF ENHANCED AXILLARY BRANCHING-DERIVED PLANTS OF TEN FOREST TREE SPECIES

V. Rani | A. Parida | S.N. Raina

110. DEVELOPMENT OF IN VITRO TECHNIQUES FOR ELIMINATION OF VIRUS DISEASES FROM MUSA

111. GROWTH CHARACTERISTICS OF MICROPROPAGATED STRAWBERRIES

S. Karhu

112. EFFECT OF SYSTEMIC FUNGICIDES ON THE DEVELOPMENT OF MICROPROPAGATED APPLE ROOTSTOCKS INOCULATED WITH MYCORRHIZAL FUNGI

G. Lubraco | A. Schubert

113. EFFECT OF IN VITRO MULTIPLICATION ON FIELD PERFORMANCE OF RASPBERRY CULTIVAR ‘NORNA’

L. Petrevica | A. Bite

114. FIELD PERFORMANCE OF IN VITRO PROPAGATED VIRUS-FREE ‘HERMOSA’ PEACH

115. HORTICULTURAL BIOTECHNOLOGY, AS EFFICIENT MEANS FOR DEVELOPING COUNTRIES SUGGESTED BY K. EREKY

M.G. Fari | E.A. Menezes | R. Bud | P.U. Kralovanszky

116. BIOTECHNOLOGY – NEW PERSPECTIVES FOR FUTURE CROP PRODUCTION

M.M. Oelck

117. TEACHING FAST AND RELIABLE PLANT TISSUE CULTURE USING PPM AND BRASSICAS

M.P. Fuller | T. Pizzey