Home > IV International Symposium on In Vitro Culture and Horticultural Breeding > GENETIC TRANSFORMATION OF SWEET POTATO (Ipomoea batatas (L.) LAM.) BY AGROBACTERIUM TUMEFACIENS

Articles

GENETIC TRANSFORMATION OF SWEET POTATO (Ipomoea batatas (L.) LAM.) BY AGROBACTERIUM TUMEFACIENS

Article number

560_35

Pages

193 – 196

Language

English

Abstract

Transgenic sweet potato plants were obtained from embryogenic calli following Agrobacterium tumefaciens-mediated transformation. A. tumefaciens strain EHA101/pIG121-Hm used in the present study contained a binary vector with genes for gusA and hygromycin resistance (hpt). Around ten hygromycin resistant cell clusters were produced from 1 g fresh weight of the infected embryogenic calli.
The hygromycin resistant plantlets were regenerated from 53.1% of the hygromycin-resistant calli.
Histochemical GUS assay and Southern hybridization analysis indicated that these plants were stably transformed with a copy number of introduced genes of one to three.
Transgenic plants grew normally and formed storage roots after 3 months of cultivation in a green house.
We have introduced some agronomically important genes by using our transformation system.

Publication

IV International Symposium on In Vitro Culture and Horticultural Breeding

Authors

M. Otani, Y. Wakita, T. Shimada

Keywords

transformation, sweet potato, Agrobacterium tumefaciens, transgenic plants

Full text

https://doi.org/10.17660/ActaHortic.2001.560.35

Online Articles (117)

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S.E. Ashmore | M. Azimi | R.A. Drew

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H. Häggman | L. Ryynänen | T. Aronen

19. CRYOPRESERVATION OF WOODY SPECIES BY VITRIFICATION OF SHOOT TIPS AND EMBRYOGENIC TISSUE

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20. TRANSFORMATION OF BEAN CALLUS BY USING HIGH-VELOCITY MICROPROJECTILES

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G. Günver

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S. Kauppinen

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M.J. Latkowska | H. Chmiel | K. Molska

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C. D'Onofrio | S. Morini

90. INFLUENCE OF GELLING AGENTS ON ILEX PARAGUARIENSIS TISSUE CULTURE

P.A. Sansberro | H.Y. Rey | C.V. Luna | L.A. Mroginski

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J. Sedlák | F. Paprstein | A. Bilavcík | J. Zámecník

92. IN VITRO PROPAGATION OF HALOXYLON APHYLLUM (MINKW.) ILJIN

Parveen | E.H. Birnbaum

93. INDUCTION AND CHARACTERIZATION OF CALLUS IN HALOXYLON APHYLLUM (MINKW.) ILJIN

A.E. Ramirez | E.H. Birnbaum

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M. Savona | L. Semeria | A. Allavena | S. Carli | P. Profumo

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D. Rzepka-Plevnes | J. Kurek

96. IN VITRO PROPAGATION OF DWARFED ROOTSTOCKS FOR SWEET CHERRY

M. Erbenová | F. Paprstein | J. Sedlák

97. IN VITRO PROPAGATION AND RECOVERY OF A HABITAT OF HABENARIA RADIATA (ORCHIDACEAE)

T. Shimada | M. Otani | M. Morii

98. ORGANOGENESIS AND ECOLOGICAL DIFFERENTIATION OF CYMBIDIUM SPECIES

K. Shimasaki | J. Tsuneishi | Y. Fukumoto

99. PAPAYA SOMATIC EMBRYO INDUCTION FROM FRUIT-BEARIN FIELD PLANTS: EFFECTS OF ROOT SUPPORTING MATERIAL AND POSITTION OF THE ROOT EXPLANTS

Jiu-Sherng Yang

100. THE EFFECT OF WOOD PROPERTIES UPON MICROPROPAGATION EFFICIENCY IN HYBRID ASPEN CLONES

101. THE ROLE OF ABA IN INDUCTION OF ANDROGENESIS IN BARLEY

S. van Bergen | R. van der Meulen | M. Wang

102. THE INFLUENCE OF GENOTYPE AND EXPLANT FOR CALLUS INDUCTION AND PROLIFERATION OF ROSA FLORIBUNDA

S. Kuusiene | M. Kandzezauskaite

103. INFLUENCE OF SONICATION ON REGENERATION AND TRANSFORMATION EFFICIENCIES IN APPLE

I. Szankowski | A. Lübke | H.-J. Jacobsen

104. SOMATIC EMBRYOGENESIS OF SILVER FIR (Abies alba Mill.) – POLISH PROVENANCES

K. Szczygiel | J. Kowalczyk

105. MICROWAVE STERILIZATION OF TISSUE CULTURE MEDIA

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106. CYCLIC SOMATIC EMBRYOGENESIS AND SCHEME FOR MULTIPLICATION OF QUERCUS ILEX L.

P.V. Mauri | J.A. Manzanera | M.M. Marcote

107. TRUE-TO TYPE PROTOCLONES REGENERATION FROM MESOPHYLL PROTOPLASTS OF “INMIL” CHERRY ROOTSTOCK (Prunus incisa x serrula)

V. Kondakova | PH. Druart

108. THE POTENTIAL FOR GENE FLOW FROM TRANSGENIC CROPS TO RELATED WILD SPECIES: A CASE STUDY IN STRAWBERRY

109. CHROMOSOME NUMBER DEPENDENT GENOME SIZE AND RAPD FINGERPRINTING DIAGNOSTICS FOR GENETIC INTEGRITY OF ENHANCED AXILLARY BRANCHING-DERIVED PLANTS OF TEN FOREST TREE SPECIES

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110. DEVELOPMENT OF IN VITRO TECHNIQUES FOR ELIMINATION OF VIRUS DISEASES FROM MUSA

111. GROWTH CHARACTERISTICS OF MICROPROPAGATED STRAWBERRIES

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112. EFFECT OF SYSTEMIC FUNGICIDES ON THE DEVELOPMENT OF MICROPROPAGATED APPLE ROOTSTOCKS INOCULATED WITH MYCORRHIZAL FUNGI

G. Lubraco | A. Schubert

113. EFFECT OF IN VITRO MULTIPLICATION ON FIELD PERFORMANCE OF RASPBERRY CULTIVAR ‘NORNA’

L. Petrevica | A. Bite

114. FIELD PERFORMANCE OF IN VITRO PROPAGATED VIRUS-FREE ‘HERMOSA’ PEACH

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M.G. Fari | E.A. Menezes | R. Bud | P.U. Kralovanszky

116. BIOTECHNOLOGY – NEW PERSPECTIVES FOR FUTURE CROP PRODUCTION

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117. TEACHING FAST AND RELIABLE PLANT TISSUE CULTURE USING PPM AND BRASSICAS

M.P. Fuller | T. Pizzey