EXPRESSION OF THE BAR AND UIDA GENES BY GLADIOLUS FOLLOWING THREE SEASONS OF DORMANCY

Gladiolus is propagated by corms that undergo dormancy each year, and at least three seasons of growth are required before the plants from tissue culture produce flowers. Successful genetic engineering of the flowering bulb crops will require long-term expression of transgenes.
Transgenic Gladiolus plants containing the bar-uidA fusion gene under control by either the CaMV 35S, rolD, mannopine synthase (mas2), or ubiquitin (UBQ3) promoters were characterized for levels of GUS expression following three seasons of dormancy.
The highest levels of GUS expression were found in callus, shoots, and roots of plants containing the CaMV 35S promoter as compared to shoots and roots from plants containing the rolD, UBQ3, and mas2 promoters.
Gene silencing of the bar and uidA genes did not occur in the 28 independently transformed plants analyzed following dormancy when both genes were expressed under the CaMV 35S, rolD, mas2 and UBQ3 promoters.

IV International Symposium on In Vitro Culture and Horticultural Breeding

K. Kamo

floral crops, corms, transgenic, monocots

https://doi.org/10.17660/ActaHortic.2001.560.28