Home > IV International Symposium on In Vitro Culture and Horticultural Breeding > GENETIC VARIABILITY OF ANTHER DONOR VERSUS SPONTANEOUS DOUBLED HAPLOID DESCENDENTS AND COLCHICINE INDUCED DOUBLED HAPLOID SWEET PEPPER (Capsicum annuum L.) LINES

Articles

GENETIC VARIABILITY OF ANTHER DONOR VERSUS SPONTANEOUS DOUBLED HAPLOID DESCENDENTS AND COLCHICINE INDUCED DOUBLED HAPLOID SWEET PEPPER (Capsicum annuum L.) LINES

Article number

560_24

Pages

149 – 152

Language

English

Abstract

Haploid (n) and doubled haploid (DH) plants were developed in anther culture of sweet pepper (Capsicum annuum L.). Regenerants were analyzed by flow cytometry for haploid (n = 12) and spontaneous doubled haploid (2n = 24) genomes.
Haploid plants were forwarded to colchicine-treatment for induced doubled haploid (2n·) plant production.
Molecular polymorphism of anther donor plants (2n), the haploid regenerants (n), the spontaneous (2n) and induced (2n·)-DH plants were analysed by RAPD-, SSR- and ISSR-PCR. The analysis of anther-donor plants compared to DH-descendents showed an unexpectedly wide range of molecular polymorphism.
Our results suggest that genetic changes occurring during meiotic recombination is higher than those of occurring during colchicine-induced genomic duplication.

Publication

IV International Symposium on In Vitro Culture and Horticultural Breeding

Authors

A. Gémesné Juhász, M. Petus, G. Venczel, L. Zatykó, G. Gyulai, M. Cséplö

Keywords

Capsicum annuum, spontaneous doubled haploid, colchicine treated doubled haploid, RAPD, SSR-ISSR PCR analysis

Full text

https://doi.org/10.17660/ActaHortic.2001.560.24

Online Articles (117)

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S. Kauppinen

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C. D'Onofrio | S. Morini

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P.A. Sansberro | H.Y. Rey | C.V. Luna | L.A. Mroginski

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J. Sedlák | F. Paprstein | A. Bilavcík | J. Zámecník

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Parveen | E.H. Birnbaum

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A.E. Ramirez | E.H. Birnbaum

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M. Savona | L. Semeria | A. Allavena | S. Carli | P. Profumo

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D. Rzepka-Plevnes | J. Kurek

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M. Erbenová | F. Paprstein | J. Sedlák

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T. Shimada | M. Otani | M. Morii

98. ORGANOGENESIS AND ECOLOGICAL DIFFERENTIATION OF CYMBIDIUM SPECIES

K. Shimasaki | J. Tsuneishi | Y. Fukumoto

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Jiu-Sherng Yang

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S. van Bergen | R. van der Meulen | M. Wang

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S. Kuusiene | M. Kandzezauskaite

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I. Szankowski | A. Lübke | H.-J. Jacobsen

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105. MICROWAVE STERILIZATION OF TISSUE CULTURE MEDIA

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P.V. Mauri | J.A. Manzanera | M.M. Marcote

107. TRUE-TO TYPE PROTOCLONES REGENERATION FROM MESOPHYLL PROTOPLASTS OF “INMIL” CHERRY ROOTSTOCK (Prunus incisa x serrula)

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108. THE POTENTIAL FOR GENE FLOW FROM TRANSGENIC CROPS TO RELATED WILD SPECIES: A CASE STUDY IN STRAWBERRY

109. CHROMOSOME NUMBER DEPENDENT GENOME SIZE AND RAPD FINGERPRINTING DIAGNOSTICS FOR GENETIC INTEGRITY OF ENHANCED AXILLARY BRANCHING-DERIVED PLANTS OF TEN FOREST TREE SPECIES

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110. DEVELOPMENT OF IN VITRO TECHNIQUES FOR ELIMINATION OF VIRUS DISEASES FROM MUSA

111. GROWTH CHARACTERISTICS OF MICROPROPAGATED STRAWBERRIES

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112. EFFECT OF SYSTEMIC FUNGICIDES ON THE DEVELOPMENT OF MICROPROPAGATED APPLE ROOTSTOCKS INOCULATED WITH MYCORRHIZAL FUNGI

G. Lubraco | A. Schubert

113. EFFECT OF IN VITRO MULTIPLICATION ON FIELD PERFORMANCE OF RASPBERRY CULTIVAR ‘NORNA’

L. Petrevica | A. Bite

114. FIELD PERFORMANCE OF IN VITRO PROPAGATED VIRUS-FREE ‘HERMOSA’ PEACH

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116. BIOTECHNOLOGY – NEW PERSPECTIVES FOR FUTURE CROP PRODUCTION

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117. TEACHING FAST AND RELIABLE PLANT TISSUE CULTURE USING PPM AND BRASSICAS

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