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Articles

CRYOPRESERVATION OF IN VITRO SUGAR BEET (Beta vulgaris) SHOOT TIPS

Article number

560_42

Pages

221 – 224

Language

English

Abstract

Sugar beet shoot tips were cryopreserved with both an encapsulation-dehydration technique and a vitrification method.
With encapsulation-dehydration a three-step preculture and dehydration to 21 % water content proved to be optimal.
When applying vitrification loading of shoot tips at 20°C for 20 to 25 min and dehydration with PVS2 at 0°C for 20 to 30 min were optimal.
With both techniques, cold acclimation clearly improved survival. Finally, vitrification is recommended to cryopreserve sugar beet shoot tips since it proved to be easier and faster and surviving shoot tips resumed growth faster.

Publication

IV International Symposium on In Vitro Culture and Horticultural Breeding

Authors

B. Vandenbussche, M. De Proft

Keywords

shoot tips, encapsulation-dehydration, vitrification, cold acclimation

Full text

https://doi.org/10.17660/ActaHortic.2001.560.42

Online Articles (117)

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