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Articles

IN VITRO ADVENTITIOUS SHOOT REGENERATION FROM VEGETATIVE APICES OF ALMOND AND OTHER PRUNUS SPECIES

Article number

560_77

Pages

403 – 406

Language

English

Abstract

A protocol for the induction of adventitious shoot formation from vegetative shoot apices of Prunus species was developed.
Adventitious shoot regeneration was obtained with this method in almond, apricot, peach and plum.
Apices, excised from in vitro shoots, were induced to regenerate for 30 days in darkness on an LP medium supplied with 8.8mM BA, 1.0mM NAA and 250mgl^-1 cefotaxime, then transferred to a fresh auxin free medium, added with 1.4mM GA₃, to the light and sub-cultured monthly.
Histological studies performed on almond M55 revealed that the adventitious shoots originated from callus.
The callus maintained shoot formation ability for months.

Publication

IV International Symposium on In Vitro Culture and Horticultural Breeding

Authors

P. Lauri, E. Caboni, C. Damiano

Keywords

Adventitious shoot regeneration, Prunus shoot culture, shoot vegetative apex.

Full text

https://doi.org/10.17660/ActaHortic.2001.560.77

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