Articles
SOMATIC EMBRYOGENESIS OF SILVER FIR (Abies alba Mill.) – POLISH PROVENANCES
Article number
560_103
Pages
509 – 512
Language
English
Abstract
Somatic embryogenesis of Abies alba Mill. was achieved according to the method of Hristoforoglu et al. (1995) with some modifications.
Mature embryos were cultured on an MCM basal medium and on a G.D. medium.
Both media were supplemented with 2.2 mM BAP, 2.3 mM kinetin, 1g/l casein hydrolysate (CH) and 500 mg/l L-glutamine, and additionally the MCM medium was used with 4.4 mM BAP. Induction frequencies using mature, zygotic embryos were 1.0-29.4 %. The immature embryos were placed on SH medium with 4.4 mM BAP or on MCM medium with 2.2 mM BAP and 2.3 mM kinetin.
The induction frequencies of embryonic suspensor mass (ESM) were 2.9-10.4 % when immature embryos were used.
For ESM proliferation, the basal medium was supplemented with 2.2 mM BAP, 2.3 mM kinetin and 0.2 mM 2,4-D with different concentrations of CH (0-1500 mg/l). Calli grew significantly better on a medium supplemented with 1500 mg/l of CH. Seasonal changes in ESM fresh weight increment were observed during the year.
For maturation, the pieces of ESM were placed on the basal medium with the concentrations of 10 to 80 mM of ± cis-trans ABA – with or without 1 mM IBA and PEG (3-7.5 %, wt 3350). The addition of 1 mM IBA and 3 % PEG into the basal medium with 60 mM ABA for cultures held in the dark improved embryo development.
The mature somatic embryos were partially dried for 2-3 weeks and then placed on the germination medium.
Germination capacity of 69-79 % was reached.
Emblings were planted to Jiffy pots or to a mixture of peat and vermiculite.
Mature embryos were cultured on an MCM basal medium and on a G.D. medium.
Both media were supplemented with 2.2 mM BAP, 2.3 mM kinetin, 1g/l casein hydrolysate (CH) and 500 mg/l L-glutamine, and additionally the MCM medium was used with 4.4 mM BAP. Induction frequencies using mature, zygotic embryos were 1.0-29.4 %. The immature embryos were placed on SH medium with 4.4 mM BAP or on MCM medium with 2.2 mM BAP and 2.3 mM kinetin.
The induction frequencies of embryonic suspensor mass (ESM) were 2.9-10.4 % when immature embryos were used.
For ESM proliferation, the basal medium was supplemented with 2.2 mM BAP, 2.3 mM kinetin and 0.2 mM 2,4-D with different concentrations of CH (0-1500 mg/l). Calli grew significantly better on a medium supplemented with 1500 mg/l of CH. Seasonal changes in ESM fresh weight increment were observed during the year.
For maturation, the pieces of ESM were placed on the basal medium with the concentrations of 10 to 80 mM of ± cis-trans ABA – with or without 1 mM IBA and PEG (3-7.5 %, wt 3350). The addition of 1 mM IBA and 3 % PEG into the basal medium with 60 mM ABA for cultures held in the dark improved embryo development.
The mature somatic embryos were partially dried for 2-3 weeks and then placed on the germination medium.
Germination capacity of 69-79 % was reached.
Emblings were planted to Jiffy pots or to a mixture of peat and vermiculite.
Authors
K. Szczygiel, J. Kowalczyk
Keywords
fir, somatic embryo, induction, proliferation, maturation
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