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Articles

TRANSFORMATION OF BEAN CALLUS BY USING HIGH-VELOCITY MICROPROJECTILES

Article number

560_19

Pages

129 – 132

Language

English

Abstract

By using hypocotyl explant and vertical culture on B5 medium containing l mg/l kinetin and 2 mg/l 2,4-D, we obtained a rapidly growing callus from bean.
We found that 25 mg/l hygromycin(Hgm) was enough to differentiate transgenic cells from the non-transgenic ones.
The GeneBooster particle delivery system was used for the bombardment of callus and the Hgm resistance gene was used as a selectable marker.
Selective test was made by using medium containing 25 and 30 mg/l Hgm to screen for transformants calli.
After selection on Hgm-containing media, several Hgm resistant growing calli were obtained.
Selective pressure was maintained over a period of 13 month for transformation tests.

Publication

IV International Symposium on In Vitro Culture and Horticultural Breeding

Authors

E. Eissa Ahmed, GY. Bisztray, I. Velich

Keywords

Phaseolus vulgaris, common bean, callus, transformation, hygromycin

Full text

https://doi.org/10.17660/ActaHortic.2001.560.19

Online Articles (117)

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C. D'Onofrio | S. Morini

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P.A. Sansberro | H.Y. Rey | C.V. Luna | L.A. Mroginski

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J. Sedlák | F. Paprstein | A. Bilavcík | J. Zámecník

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Parveen | E.H. Birnbaum

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A.E. Ramirez | E.H. Birnbaum

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M. Savona | L. Semeria | A. Allavena | S. Carli | P. Profumo

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D. Rzepka-Plevnes | J. Kurek

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M. Erbenová | F. Paprstein | J. Sedlák

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T. Shimada | M. Otani | M. Morii

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K. Shimasaki | J. Tsuneishi | Y. Fukumoto

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S. van Bergen | R. van der Meulen | M. Wang

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S. Kuusiene | M. Kandzezauskaite

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