Articles
IN VITRO CULTURE OF HUNGARIAN APRICOT (Prunus armeniaca L.) VARIETIES
Article number
560_75
Pages
395 – 398
Language
English
Abstract
Apricot is one of the most important fruit crops in Hungary, with a production of 40000 t/year.
Hungary, like most of the Europian countries is strongly infected with Plum pox virus.
The virus elimination till now happened by thermotherapy in vivo.
This method is very effective, but time-consuming.
To shorten the time of virus elimination the in vitro thermotherapy is planned, as first step to it the development of sterile propagation method of the apricot varieties.
Actively growing shoot-tips of the required varieties were collected for initiation of the culture.
Following a desinfection procedure 70 % of the shoots begin to grow under sterile condition.
The growing shoots have to be transferred into fresh propagation medium every 3 weeks and kept in a growth room of 22 °C, under 16/8 h photoperiod with a light intensity of 1500 Lux.
The typical propagation rate of the different varieties is reached 3-4 months following the start of induction, differing from 3 to 8. An elongation medium with a relatively high IAA concentration is neccesary to use before rooting.
The elongated, about 20 mm long shoots should be rooted on a half strength modified medium.
The rooted shoots can be acclimatized under high humidity to the greenhouse condition.
The development of the micropropagation method of different apricot varieties is a good opportunity to begin the in vitro thermotherapy trials to get virusfree plants as well as chould be a basis for regeneration experiments in genetic transformation against Plum pox virus.
Hungary, like most of the Europian countries is strongly infected with Plum pox virus.
The virus elimination till now happened by thermotherapy in vivo.
This method is very effective, but time-consuming.
To shorten the time of virus elimination the in vitro thermotherapy is planned, as first step to it the development of sterile propagation method of the apricot varieties.
Actively growing shoot-tips of the required varieties were collected for initiation of the culture.
Following a desinfection procedure 70 % of the shoots begin to grow under sterile condition.
The growing shoots have to be transferred into fresh propagation medium every 3 weeks and kept in a growth room of 22 °C, under 16/8 h photoperiod with a light intensity of 1500 Lux.
The typical propagation rate of the different varieties is reached 3-4 months following the start of induction, differing from 3 to 8. An elongation medium with a relatively high IAA concentration is neccesary to use before rooting.
The elongated, about 20 mm long shoots should be rooted on a half strength modified medium.
The rooted shoots can be acclimatized under high humidity to the greenhouse condition.
The development of the micropropagation method of different apricot varieties is a good opportunity to begin the in vitro thermotherapy trials to get virusfree plants as well as chould be a basis for regeneration experiments in genetic transformation against Plum pox virus.
Authors
I. Balla, J. Vértesy
Keywords
micropropagation, apricot varieties, virus elimination, BAP-ribosid, elongation
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