Articles
AGROBACTERIUM-MEDIATED TRANSIENT EXPRESSION OF β-GLUCURONIDASE AND LUCIFERASE GENES IN LIMONIUM SINUATUM
Article number
560_32
Pages
181 – 184
Language
English
Abstract
We have developed an Agrobacterium-mediated transformation system for Limonium sinuatum. The shoot regeneration was observed on thidiazuron (TDZ)-containing medium.
Shoot-forming capacity of this species depended on the cultivar.
About 40 % of the explants of’ cv.
PF142 regenerated shoots on 0.1 µM TDZ medium.
Luciferase (LUC) and
-glucuronidase (GUS) genes were introduced and transient expression of these gene were confirmed.
Stable expression of the GUS gene was also confirmed, but shoot formation was strongly inhibited by hygromycin B, of which resistant gene was used as a selection marker.
Shoot-forming capacity of this species depended on the cultivar.
About 40 % of the explants of’ cv.
PF142 regenerated shoots on 0.1 µM TDZ medium.
Luciferase (LUC) and
-glucuronidase (GUS) genes were introduced and transient expression of these gene were confirmed.Stable expression of the GUS gene was also confirmed, but shoot formation was strongly inhibited by hygromycin B, of which resistant gene was used as a selection marker.
Authors
M. Kimizou, Y. Yamamoto, T. Iwasaki, S. Ohki
Keywords
statice, Thidiazuron, leaf explant, hygromycin resistance, intron
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