Articles
Factors with major effects for differentiation and regeneration processes from tomato anther culture
Article number
1439_20
Pages
145 – 152
Language
English
Abstract
The aim of this study was to analyze the potential of dedifferentiation and regeneration from anther culture with five tomato genotypes (‘Argeș 20’, ‘Argeș 11’, ‘Ștefănești 22’, ‘Ștefănești 24’, and ‘Costate 21’). The outcome of anther culture is strongly influenced by different factors such as plant genotype, bud, and anther sizes, the viability of pollen microspores and their stage of development at the moment of inoculation, and culture media composition for different in vitro stages of tissue development.
According to our measurements, microscope, and visual observations, as of the statistical analysis of the results, the following conclusions were drawn: a) flower buds of 4-8 mm size containing anthers of 2.5-5 mm long had the best responses during in vitro culture; b) the anthers containing microspores at late meiosis to first mitosis stages were the most efficient for further on regenerative processes; c) the culture medium containing K (0.5 mg L-1) and IAA (0.5 mg L-1) proved to ensure the best results for anthers viability and organogenic structures regeneration, while the media containing BAP (2.0 mg L-1) and 2,4D (2.0 mg L-1) was the most efficient for the callus induction and its growth; d) a strong genotypic response was found among tomato cultivars: callusogenesis was obtained with all tested genotypes and organogenesis processes only with ‘Argeș 20’ and ‘Costate 21’.
According to our measurements, microscope, and visual observations, as of the statistical analysis of the results, the following conclusions were drawn: a) flower buds of 4-8 mm size containing anthers of 2.5-5 mm long had the best responses during in vitro culture; b) the anthers containing microspores at late meiosis to first mitosis stages were the most efficient for further on regenerative processes; c) the culture medium containing K (0.5 mg L-1) and IAA (0.5 mg L-1) proved to ensure the best results for anthers viability and organogenic structures regeneration, while the media containing BAP (2.0 mg L-1) and 2,4D (2.0 mg L-1) was the most efficient for the callus induction and its growth; d) a strong genotypic response was found among tomato cultivars: callusogenesis was obtained with all tested genotypes and organogenesis processes only with ‘Argeș 20’ and ‘Costate 21’.
Authors
A.E. Alexandrescu (Manolescu), C.F. Popescu, A. Popescu, A.M. Dumitru, D.I. Sumedrea, S.N. Cosmulescu
Keywords
androgenesis, callus, in vitro culture, microspores, Solanum lycopersicum
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