Articles
Micropropagation of Origanum vulgare without growth regulators
Article number
1439_42
Pages
319 – 326
Language
English
Abstract
Origanum vulgare L. is an important aromatic and pharmaceutical native to Greece.
Growers and nurseries select natural populations with additional aromatic and pharmaceutical characteristics, propagate and cultivate them.
Micropropagation can be used as a technique for propagating selected plants and populations and produce plants genetically similar with the parent’s plants.
At the present study the possibility to micro propagates Origanum vulgare plants without using plant growth regulators examined.
Sprouts of nodal shoots were used, which were cut from native plants from the mountain Taygetus.
The explants were placed on half strength MS substrates with 1.5% sucrose, and the culture plates were covered with semipermeable membrane or parafilm.
Explants establish at the beginning, at the middle and at the end of the germination period and explants origin from the lower or the upper part of the shoot.
A higher rate of proliferation was achieved when explants originated from the upper part of the parent shoot at the beginning of the germination period.
In the proliferation cultures when explants originated from the top of the shoots higher rates of proliferation achieved, while a high percentage of shoots formed roots at the same time.
The use of a semi-permeable membrane as a covering material of the culture plates increased the rate of proliferation and increased the acclimatization of the seedlings to ex vitro conditions.
Growers and nurseries select natural populations with additional aromatic and pharmaceutical characteristics, propagate and cultivate them.
Micropropagation can be used as a technique for propagating selected plants and populations and produce plants genetically similar with the parent’s plants.
At the present study the possibility to micro propagates Origanum vulgare plants without using plant growth regulators examined.
Sprouts of nodal shoots were used, which were cut from native plants from the mountain Taygetus.
The explants were placed on half strength MS substrates with 1.5% sucrose, and the culture plates were covered with semipermeable membrane or parafilm.
Explants establish at the beginning, at the middle and at the end of the germination period and explants origin from the lower or the upper part of the shoot.
A higher rate of proliferation was achieved when explants originated from the upper part of the parent shoot at the beginning of the germination period.
In the proliferation cultures when explants originated from the top of the shoots higher rates of proliferation achieved, while a high percentage of shoots formed roots at the same time.
The use of a semi-permeable membrane as a covering material of the culture plates increased the rate of proliferation and increased the acclimatization of the seedlings to ex vitro conditions.
Authors
S. Karras, E. Kartsonas, A. Alexopoulos, A. Darras, I. Lykoskoufis
Keywords
micropropagation, native, aromatic, acclimatization, photoreceptor, in vitro plant
Online Articles (75)