Articles
Identification of the main bioactive compounds in sideritis genotypes Genebanked at BRGV Buzau
Article number
1439_52
Pages
403 – 410
Language
English
Abstract
The study of Sideritis species has intensified in recent years.
Their undeniable value as aromatic, therapeutic, ornamental, and melliferous plant, along with the restriction of the cultivation area due to massive harvesting and climate changes, has captured the interest of the scientific community, focusing on advancing botanical, phytotherapeutic, and biochemical research.
BRGV Buzau has paid special attention to Sideritis species; therefore, a large number of genotypes in various stages of breeding are currently biobanked here, including S. scardica Griseb., S. syriaca L., S. hyssopifolia L., S. scardica ‘Ossa’, S. cypria Post, and S. glacialis Boiss.
The research undertaken on the main genetically stabilized genotypes has focused on acclimatization, breeding, and the development of specific culture technologies, as well as on determining the biochemical composition.
The genotypes examined in this study include G1 – S. syriaca L., G2 – S. scardica Griseb., and G3 – S. hyssopifolia L. Samples of the aerial parts collected in two successive years, 2022 and 2023, were analyzed, and the following compounds were determined: volatile compounds by using the GC-MS technique, antioxidant activity using the DPPH method, and the total content of phenols using the Folin-Ciocâlteu method.
No major differences were found in the total phenol content, with the highest amount (9.17±0.32 mg GAE g‑1 DM) being recorded for the species S. hyssopifolia.
However, S. scardica exhibited higher antioxidant activity (96.14±5.57 mg TE g‑1 DM) compared to the other two species.
Regarding the composition of the essential oils, the fresh sample material showed the presence of a high percentage of monoterpenes, sesquiterpenes, oxygenated monoterpenes, and diterpenes.
The chemical composition varied from 18.37%
-pinene in S. scardica to 11.24% in S. syriaca and 25.38% in S. hyssopifolia. The conducted research allows a more precise expansion toward new directions of use of Sideritis species, compared to the previously known applications.
While in the past they were valued for their aromatic and food-related properties, the obtained results certify the therapeutic and medicinal properties of these species, justifying their use for the benefit of human health.
Their undeniable value as aromatic, therapeutic, ornamental, and melliferous plant, along with the restriction of the cultivation area due to massive harvesting and climate changes, has captured the interest of the scientific community, focusing on advancing botanical, phytotherapeutic, and biochemical research.
BRGV Buzau has paid special attention to Sideritis species; therefore, a large number of genotypes in various stages of breeding are currently biobanked here, including S. scardica Griseb., S. syriaca L., S. hyssopifolia L., S. scardica ‘Ossa’, S. cypria Post, and S. glacialis Boiss.
The research undertaken on the main genetically stabilized genotypes has focused on acclimatization, breeding, and the development of specific culture technologies, as well as on determining the biochemical composition.
The genotypes examined in this study include G1 – S. syriaca L., G2 – S. scardica Griseb., and G3 – S. hyssopifolia L. Samples of the aerial parts collected in two successive years, 2022 and 2023, were analyzed, and the following compounds were determined: volatile compounds by using the GC-MS technique, antioxidant activity using the DPPH method, and the total content of phenols using the Folin-Ciocâlteu method.
No major differences were found in the total phenol content, with the highest amount (9.17±0.32 mg GAE g‑1 DM) being recorded for the species S. hyssopifolia.
However, S. scardica exhibited higher antioxidant activity (96.14±5.57 mg TE g‑1 DM) compared to the other two species.
Regarding the composition of the essential oils, the fresh sample material showed the presence of a high percentage of monoterpenes, sesquiterpenes, oxygenated monoterpenes, and diterpenes.
The chemical composition varied from 18.37%
-pinene in S. scardica to 11.24% in S. syriaca and 25.38% in S. hyssopifolia. The conducted research allows a more precise expansion toward new directions of use of Sideritis species, compared to the previously known applications.While in the past they were valued for their aromatic and food-related properties, the obtained results certify the therapeutic and medicinal properties of these species, justifying their use for the benefit of human health.
Authors
G. Negoșanu, C. Vînătoru, V.A. Ion, B. Mușat, M. Popescu, A. Asănică
Keywords
acclimatization, chemical composition, essential oils, germplasm, phenotype
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