Articles
The effect of different selective reagents on in vitro shoot regeneration in bigleaf hortensias (Hydrangea macrophylla)
Article number
1439_30
Pages
227 – 234
Language
English
Abstract
Bigleaf hortensias (Hydrangea macrophylla) are perennial, woody plants of the family Hydrangeaceae.
They are native to East and Southeast Asia, and are nowadays cultivated as ornamental plants worldwide.
Breeding of bigleaf hortensias is challenging because of long generation cycles, sexual barriers and polyploidy.
Here, genetic engineering is of interest to introduce new and desired traits while maintaining the genetic background of highly accepted varieties.
Mostly, a selection system is required to select genetically modified plants after Agrobacterium-mediated transformation.
The kanamycin/nptII-based selection system has already been successfully used to produce transgenic H. macrophylla plants.
Additional selection systems would enable further applications such as selection of plants after combining different transgenes through retransformation or crossing.
In order to identify additional selection systems, we studied the phytotoxicity of kanamycin, gentamicin, hygromycin B and ammonium glyphosate on the in vitro shoot regeneration of leaf explants of H. macrophylla cultivar ‘Blaumeise’. All tested reagents showed inhibitory effects on vitality, callus formation and shoot regeneration.
Kanamycin and gentamicin were moderate-toxic and caused delayed death of plant tissue.
In contrast, hygromycin B and ammonium glyphosate showed strong phytotoxic effects even at low concentrations.
A complete inhibition of shoot regeneration was observed at minimum concentrations of 50 mg L-1 kanamycin, 70 mg L-1 gentamicin, 5 mg L-1 hygromycin B and at all ammonium glyphosate concentrations tested (3-50 mg L-1).
They are native to East and Southeast Asia, and are nowadays cultivated as ornamental plants worldwide.
Breeding of bigleaf hortensias is challenging because of long generation cycles, sexual barriers and polyploidy.
Here, genetic engineering is of interest to introduce new and desired traits while maintaining the genetic background of highly accepted varieties.
Mostly, a selection system is required to select genetically modified plants after Agrobacterium-mediated transformation.
The kanamycin/nptII-based selection system has already been successfully used to produce transgenic H. macrophylla plants.
Additional selection systems would enable further applications such as selection of plants after combining different transgenes through retransformation or crossing.
In order to identify additional selection systems, we studied the phytotoxicity of kanamycin, gentamicin, hygromycin B and ammonium glyphosate on the in vitro shoot regeneration of leaf explants of H. macrophylla cultivar ‘Blaumeise’. All tested reagents showed inhibitory effects on vitality, callus formation and shoot regeneration.
Kanamycin and gentamicin were moderate-toxic and caused delayed death of plant tissue.
In contrast, hygromycin B and ammonium glyphosate showed strong phytotoxic effects even at low concentrations.
A complete inhibition of shoot regeneration was observed at minimum concentrations of 50 mg L-1 kanamycin, 70 mg L-1 gentamicin, 5 mg L-1 hygromycin B and at all ammonium glyphosate concentrations tested (3-50 mg L-1).
Authors
C. Tränkner, L. Carter, L.M. Montejano Rodriguez
Keywords
antibiotic, biotechnology, genetic engineering, herbicide, in vitro, ornamental
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