Articles
In vitro establishment and micrografting essay for Portuguese olive (Olea europaea L.) cultivars
Article number
1439_33
Pages
253 – 260
Language
English
Abstract
The olive tree (Olea europaea L.) stands as a cornerstone of socio-economic, nutritional, and environmental significance in the Mediterranean region.
Traditionally, this species is vegetatively propagated through cuttings, or grafting onto seedlings.
Despite extensive attempts to enhance self-rooting success, many cultivars still showing recalcitrance, making grafting the only solution for vegetative propagation.
To address the challenges associated with olive propagation limitations, this work proposes a strategic shift toward the application of biotechnological techniques, particularly in vitro micropropagation and in vitro micrografting (IVM). In this work, four Portuguese olive cultivars characterized by medium/low vigor: Galega vulgar, Blanqueta da Elvas, Azeiteira, and Cobrancosa, were propagated in vitro and survival rates were recorded.
The preeminent rate was observed in a specific genotype of Galega vulgar, boasting a remarkable 75% of survival rate accompanied by successful subcultures.
Subsequently, Cobrancosa registered a commendable 55% survival rate and the Azeiteira conversely demonstrated the lowest recorded survival rate 33%. According to these findings, Galega vulgar was advantageously chosen for further investigation, specifically to explore the impact of substituting zeatin with coconut water in the shooting culture medium and to test the initial in vitro micrografting (IVM) assay.
The primary objective of the initial IVM assessment was to study the morphological variations (wounding, callus formation and vascular connection) ensuing from the micrografting process within Galega vulgar.
Traditionally, this species is vegetatively propagated through cuttings, or grafting onto seedlings.
Despite extensive attempts to enhance self-rooting success, many cultivars still showing recalcitrance, making grafting the only solution for vegetative propagation.
To address the challenges associated with olive propagation limitations, this work proposes a strategic shift toward the application of biotechnological techniques, particularly in vitro micropropagation and in vitro micrografting (IVM). In this work, four Portuguese olive cultivars characterized by medium/low vigor: Galega vulgar, Blanqueta da Elvas, Azeiteira, and Cobrancosa, were propagated in vitro and survival rates were recorded.
The preeminent rate was observed in a specific genotype of Galega vulgar, boasting a remarkable 75% of survival rate accompanied by successful subcultures.
Subsequently, Cobrancosa registered a commendable 55% survival rate and the Azeiteira conversely demonstrated the lowest recorded survival rate 33%. According to these findings, Galega vulgar was advantageously chosen for further investigation, specifically to explore the impact of substituting zeatin with coconut water in the shooting culture medium and to test the initial in vitro micrografting (IVM) assay.
The primary objective of the initial IVM assessment was to study the morphological variations (wounding, callus formation and vascular connection) ensuing from the micrografting process within Galega vulgar.
Authors
N. Jouini, E. Baltazar, T. Lopes, M. Correia, M.A. Germanà, J.M. Canhoto, T. Caruso
Keywords
grafting union, histological analysis, plant tissue culture, recalcitrance
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