Articles
Transcriptomic analysis of water-stressed tomato (Solanum lycopersicum L.) treated with yeast extracts and algae-based biostimulant
Article number
1439_71
Pages
535 – 542
Language
English
Abstract
Water deficit is one of the most pressing environmental stresses globally.
In this context, the use of biostimulants represents a sustainable practice aimed at improving crop tolerance and mitigating negative effects on productivity.
In this work, the effects of ERANTHIS®, a biostimulant based on seaweed (Ascophyllum nodosum and Laminaria digitata) and yeast extracts, were analyzed on tomato (Solanum lycopersicum L. ‘Micro-Tom’) grown under drought stress.
Morphometric and biochemical parameters were evaluated at two experimental time points (5 and 24 h after the third biostimulant treatment). H2O2, osmolytes (proline and glycine betaine), non-protein thiol content, and antioxidant enzymes (SOD, CAT, POX, and GST) activity were measured in leaves along with the level of transcripts using the RNA-seq technique through Illumina sequencing and the DESeq2 pipeline.
Comparison of samples under different experimental conditions allowed the identification of some genes differentially expressed following treatment with ERANTHIS®. Overall, some gene functions were observed to be overexpressed (e.g. chitin degradation, cell wall expansion, and hormone activity) and underexpressed (e.g. photosynthetic processes, lipid metabolism). These data will provide a better understanding of how the biostimulant ERANTHIS® acts in mitigating the effects of water stress.
In this context, the use of biostimulants represents a sustainable practice aimed at improving crop tolerance and mitigating negative effects on productivity.
In this work, the effects of ERANTHIS®, a biostimulant based on seaweed (Ascophyllum nodosum and Laminaria digitata) and yeast extracts, were analyzed on tomato (Solanum lycopersicum L. ‘Micro-Tom’) grown under drought stress.
Morphometric and biochemical parameters were evaluated at two experimental time points (5 and 24 h after the third biostimulant treatment). H2O2, osmolytes (proline and glycine betaine), non-protein thiol content, and antioxidant enzymes (SOD, CAT, POX, and GST) activity were measured in leaves along with the level of transcripts using the RNA-seq technique through Illumina sequencing and the DESeq2 pipeline.
Comparison of samples under different experimental conditions allowed the identification of some genes differentially expressed following treatment with ERANTHIS®. Overall, some gene functions were observed to be overexpressed (e.g. chitin degradation, cell wall expansion, and hormone activity) and underexpressed (e.g. photosynthetic processes, lipid metabolism). These data will provide a better understanding of how the biostimulant ERANTHIS® acts in mitigating the effects of water stress.
Authors
P. Cerruti, C. Campobenedetto, C. Agliassa, V. Contartese, E. Montrucchio, M. Martina, C. Bertea, A. Acquadro
Keywords
biostimulant, RNA-seq, drought stress, tomato
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