Articles
In vitro seed germination of wild strawberry, through chemical scarification and carbon-rich compounds addition in culture medium
Article number
1439_34
Pages
261 – 266
Language
English
Abstract
Low and slow germination of wild strawberry (Fragaria vesca L.) seeds is affecting the success of breeding programs.
Pretreating seeds, before sowing, is mandatory for some wild strawberry genotypes.
Setting up a valid and efficient in vitro seed germination protocol can further speed up breeding programs, allowing a more precise control of environmental and growing condition, but also making the process independent of the seasonality.
In the last years, a great interest is rising about circular economy, and, also, in plant tissue culture, compounds derived from agri-food waste, such as biochar, are starting to be used, exploiting their potential in improving explant performances.
In this study, the effect of chemical scarification on the gemination of two cultivars (‘Ali-Baba’ and ‘Reugen’) of wild strawberry (Fragaria vesca L.) seeds cultured in vitro, on media enriched with biochar and activated charcoal (4 and 6 g L‑1) was studied.
Before sowing, half of the seeds were treated with H2SO4, for 5 min; afterwards, all seeds, treated and not, were put in culture in the different culture media.
At the end of experiment, different parameters were analyzed, germinability (FGP), mean germination time (MGT) and germination value (GV) to evaluate the effect of the factors tested (cultivar, chemical scarification, and culture medium composition) on wild strawberry seed germination.
Results obtained evidenced that the chemical scarification is the only factor that could markedly improve the germination process, while the presence either of biochar or activated charcoal did not seem to affect the seed behavior.
This study put the basis to further investigate different treatments to improve in vitro seed germination of wild strawberry, but also to evaluate compounds from agrifood waste, like the biochar, as culture medium components.
Pretreating seeds, before sowing, is mandatory for some wild strawberry genotypes.
Setting up a valid and efficient in vitro seed germination protocol can further speed up breeding programs, allowing a more precise control of environmental and growing condition, but also making the process independent of the seasonality.
In the last years, a great interest is rising about circular economy, and, also, in plant tissue culture, compounds derived from agri-food waste, such as biochar, are starting to be used, exploiting their potential in improving explant performances.
In this study, the effect of chemical scarification on the gemination of two cultivars (‘Ali-Baba’ and ‘Reugen’) of wild strawberry (Fragaria vesca L.) seeds cultured in vitro, on media enriched with biochar and activated charcoal (4 and 6 g L‑1) was studied.
Before sowing, half of the seeds were treated with H2SO4, for 5 min; afterwards, all seeds, treated and not, were put in culture in the different culture media.
At the end of experiment, different parameters were analyzed, germinability (FGP), mean germination time (MGT) and germination value (GV) to evaluate the effect of the factors tested (cultivar, chemical scarification, and culture medium composition) on wild strawberry seed germination.
Results obtained evidenced that the chemical scarification is the only factor that could markedly improve the germination process, while the presence either of biochar or activated charcoal did not seem to affect the seed behavior.
This study put the basis to further investigate different treatments to improve in vitro seed germination of wild strawberry, but also to evaluate compounds from agrifood waste, like the biochar, as culture medium components.
Authors
S. Nazeer, A. Agosti, L. Leto, B. Chiancone
Keywords
activated charcoal, biochar, Fragaria vesca L., germinability, sulphuric acid
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