Articles
Functional characterization of PavCYP78As affecting sweet cherry fruit size
Article number
1408_20
Pages
151 – 156
Language
English
Abstract
Sweet cherry (Prunus avium L.) fruit size has long been one of the most important indicators for consumers selection, and also directly determines the yield and economic benefits.
Understanding the genetic mechanisms underlying the control of sweet cherry fruit size is essential for breeding new varieties.
However, to date, few genes related to the molecular mechanisms have been identified.
In the present study, the molecular functions of several PavCYP78A family members, PavCYP78A9, PavCYP78A6, and PavCYP78A5/KLUH regulating sweet cherry fruit size were characterized. PavCYP78A9 and PavCYP78A6 regulated the fruit size by promoting cell proliferation cell proliferation and expansion during early fruit growth and development, and PavCYP78A6 in sweet cherry is functionally redundant with PavCYP78A9 affecting fruit size. PavKLUH gene regulated fruit size by involving in cell expansion.
Furthermore, PavAGL15, an agamous-like MADS-box transcription factor, directly repressed PavCYP78A9 expression to influence fruit size by Yeast one-hybrid (Y1H) and transient dual-luciferase reporter assays (LUC). Similarly, an AP2/ERF transcription factor PavRAV2 negatively modulated fruit size by directly repressing PavKLUH expression.
In conclusion, we investigated the role of CYP78A family members in sweet cherry fruit size regulation, and our findings provided with novel insights to understand the molecular mechanisms underlying fruit size determination during fruit growth and development in fruit trees.
Understanding the genetic mechanisms underlying the control of sweet cherry fruit size is essential for breeding new varieties.
However, to date, few genes related to the molecular mechanisms have been identified.
In the present study, the molecular functions of several PavCYP78A family members, PavCYP78A9, PavCYP78A6, and PavCYP78A5/KLUH regulating sweet cherry fruit size were characterized. PavCYP78A9 and PavCYP78A6 regulated the fruit size by promoting cell proliferation cell proliferation and expansion during early fruit growth and development, and PavCYP78A6 in sweet cherry is functionally redundant with PavCYP78A9 affecting fruit size. PavKLUH gene regulated fruit size by involving in cell expansion.
Furthermore, PavAGL15, an agamous-like MADS-box transcription factor, directly repressed PavCYP78A9 expression to influence fruit size by Yeast one-hybrid (Y1H) and transient dual-luciferase reporter assays (LUC). Similarly, an AP2/ERF transcription factor PavRAV2 negatively modulated fruit size by directly repressing PavKLUH expression.
In conclusion, we investigated the role of CYP78A family members in sweet cherry fruit size regulation, and our findings provided with novel insights to understand the molecular mechanisms underlying fruit size determination during fruit growth and development in fruit trees.
Publication
Authors
X.L. Qi, C.L. Liu, L.L. Song, Y.X. Dong, L. Chen, M. Li
Keywords
sweet cherry, CYP78A, fruit size, silencing, overexpression, promoter
Groups involved
Online Articles (71)