Articles
Multi-generation selfing produced the sweet cherry germplasm with the increased fruit firmness and reduced heterozygosity
Article number
1408_21
Pages
157 – 164
Language
English
Abstract
The sweet cherry (Prunus avium L.) is an important economic tree species, known as the “king of fruit”. Due to the availability of self-fertile materials and the progress of high-throughput sequencing technology, it is possible to study the molecular and genetic basis of genomics changes of sweet cherry after multiple generations of selfing.
Here, we measured the genomic changes of sweet cherry self-compatible cultivar ‘Stella’ for three consecutive generations through the second generation re-sequencing, and found that there was no significant change in the size of the genome and the proportion of repeat sequences through multiple generations of self-pollination, but the heterozygosity was significantly reduced.
At the same time, we measured the metabolic changes of the fruit after three generations of self-pollination, and found fruit composition changed significantly.
In terms of its commercial properties, its size and shape did not change significantly, but its firmness changed significantly.
The low heterozygosity materials obtained in this study have laid a foundation for the study of sweet cherry genomics.
The changes of the genomes and fruit characters of the selected sweet cherry after multiple generations of self-pollination provide us with new clues for the directional improvement of fruit tree quality, that is, fruit trees genotypes can be purified through multiple generations of self-pollination and target characters and genotypes also can be fixed, laying a material foundation for further research on multi trait aggregation.
Here, we measured the genomic changes of sweet cherry self-compatible cultivar ‘Stella’ for three consecutive generations through the second generation re-sequencing, and found that there was no significant change in the size of the genome and the proportion of repeat sequences through multiple generations of self-pollination, but the heterozygosity was significantly reduced.
At the same time, we measured the metabolic changes of the fruit after three generations of self-pollination, and found fruit composition changed significantly.
In terms of its commercial properties, its size and shape did not change significantly, but its firmness changed significantly.
The low heterozygosity materials obtained in this study have laid a foundation for the study of sweet cherry genomics.
The changes of the genomes and fruit characters of the selected sweet cherry after multiple generations of self-pollination provide us with new clues for the directional improvement of fruit tree quality, that is, fruit trees genotypes can be purified through multiple generations of self-pollination and target characters and genotypes also can be fixed, laying a material foundation for further research on multi trait aggregation.
Publication
Authors
X. Zhang, X.W. Duan, G.H. Yan, X.M. Zhang, J. Wang, Y. Zhou, C.B. Wu, Y.L. Li, K.C. Zhang
Keywords
Prunus avium L., selfing, heterozygosity, firmness
Groups involved
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