Articles
Effects of combined high temperature and waterlogging stresses on physiological and biochemical characteristics of root system in Cerasus sachalinensis Kom.
Article number
1408_65
Pages
485 – 494
Language
English
Abstract
Cherry cultivation often encounters various environmental stresses such as high temperature and waterlogging in summer.
In order to explore the damage mechanism of these stresses on plant growth and development, seedlings of Cerasus sachalinensis were treated with high temperature, waterlogging and their combination to study in particular the physiological and biochemical responses related to root respiration and active oxygen metabolism.
The results showed that, both high temperature and waterlogging stresses caused a decrease in total root respiration rate, tricarboxylic acid cycling pathway (TCA) and pentose phosphate pathway (PPP), while the respiration rate of glycolysis pathway (EMP) increased first and then decreased.
The damage to root function was more serious under the combined stress, and the total respiration rate of the root system was significantly lower than under any single stress.
The contents of malondialdehyde (MDA), superoxide anion (O2·–) and hydrogen peroxide (H2O2) in the root system of C. sachalinensis increased under single high temperature and waterlogging stresses; the activities of guaiacol peroxidase (POD), catalase (CAT) and ascorbate peroxidase (APX), and the contents of ascorbic acid (AsA), monodehydroascorbic (DHA), reduced glutathione (GSH) and oxidized glutathione (GSSG) all increased first and then decreased.
The trend of reactive oxygen accumulation and metabolism under combined stresses was consistent with that under single stress, but the peak value of reactive oxygen accumulation was significantly higher than that under any single stress.
In addition, the peak value of the activities of POD, CAT and APX, and the content of AsA, DHA, GSH and GSSG were significantly higher under combined stresses than that under any single stress.
Combined stresses further intensified the accumulation of reactive oxygen species and stimulated the reactive oxygen metabolism system.
In order to explore the damage mechanism of these stresses on plant growth and development, seedlings of Cerasus sachalinensis were treated with high temperature, waterlogging and their combination to study in particular the physiological and biochemical responses related to root respiration and active oxygen metabolism.
The results showed that, both high temperature and waterlogging stresses caused a decrease in total root respiration rate, tricarboxylic acid cycling pathway (TCA) and pentose phosphate pathway (PPP), while the respiration rate of glycolysis pathway (EMP) increased first and then decreased.
The damage to root function was more serious under the combined stress, and the total respiration rate of the root system was significantly lower than under any single stress.
The contents of malondialdehyde (MDA), superoxide anion (O2·–) and hydrogen peroxide (H2O2) in the root system of C. sachalinensis increased under single high temperature and waterlogging stresses; the activities of guaiacol peroxidase (POD), catalase (CAT) and ascorbate peroxidase (APX), and the contents of ascorbic acid (AsA), monodehydroascorbic (DHA), reduced glutathione (GSH) and oxidized glutathione (GSSG) all increased first and then decreased.
The trend of reactive oxygen accumulation and metabolism under combined stresses was consistent with that under single stress, but the peak value of reactive oxygen accumulation was significantly higher than that under any single stress.
In addition, the peak value of the activities of POD, CAT and APX, and the content of AsA, DHA, GSH and GSSG were significantly higher under combined stresses than that under any single stress.
Combined stresses further intensified the accumulation of reactive oxygen species and stimulated the reactive oxygen metabolism system.
Publication
Authors
Z. Tao, M.Q. He, Q.Y. Zhang, J.L. He, S.J. Qin
Keywords
Cerasus sachalinensis Kom., compound stress, respiratory metabolism, reactive oxygen metabolism
Groups involved
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