Articles
Cloning and functional analysis of PavbHLH13 in sweet cherry
Article number
1408_25
Pages
181 – 190
Language
English
Abstract
bHLH transcription factors play an important role in regulating plant growth and development, signal transduction, and stress resistance.
To understand the regulatory effect of bHLH transcription factors on the coloration of sweet cherry fruit, the full-length CDS sequence of the PavbHLH13 gene was cloned from the sweet cherry cultivar ‘Tieton’ and analyzed using bioinformatics tools.
The tissue-specific expression of the gene was analyzed using RT-PCR, and the function of the gene in fruit coloration was analyzed using virus induced gene silencing (VIGS). The full-length CDS of PavbHLH13 was 1719 bp, encoding a protein of 572 amino acids.
The promoter region of PavbHLH13 contained a variety of elements relating to light response, hormone response, and signal transduction. PavbHLH13 expression was highest in ripe fruit, second highest in leaves, and lowest in young fruit.
High temperatures inhibited the expression of this gene in fruit peel.
At the color-change stage during fruit development, TRV::bHLH was used to inoculate sweet cherry fruit, which significantly lowered the expression of PavbHLH13 and the structural genes for anthocyanin synthesis in the fruit peel compared with that in the control group.
At 10 d after inoculation, the inoculation site was still light yellow-green, while the fruit peel of the control was red.
The results indicated that the PavbHLH13 gene was involved in regulating the fruit coloration of sweet cherry, and its expression level was regulated by temperature.
To understand the regulatory effect of bHLH transcription factors on the coloration of sweet cherry fruit, the full-length CDS sequence of the PavbHLH13 gene was cloned from the sweet cherry cultivar ‘Tieton’ and analyzed using bioinformatics tools.
The tissue-specific expression of the gene was analyzed using RT-PCR, and the function of the gene in fruit coloration was analyzed using virus induced gene silencing (VIGS). The full-length CDS of PavbHLH13 was 1719 bp, encoding a protein of 572 amino acids.
The promoter region of PavbHLH13 contained a variety of elements relating to light response, hormone response, and signal transduction. PavbHLH13 expression was highest in ripe fruit, second highest in leaves, and lowest in young fruit.
High temperatures inhibited the expression of this gene in fruit peel.
At the color-change stage during fruit development, TRV::bHLH was used to inoculate sweet cherry fruit, which significantly lowered the expression of PavbHLH13 and the structural genes for anthocyanin synthesis in the fruit peel compared with that in the control group.
At 10 d after inoculation, the inoculation site was still light yellow-green, while the fruit peel of the control was red.
The results indicated that the PavbHLH13 gene was involved in regulating the fruit coloration of sweet cherry, and its expression level was regulated by temperature.
Publication
Authors
Y. Tan, L. Xu, X.J. Zong, Q. Niu, Y.G. Sun, H.R. Wei
Keywords
sweet cherry, bHLH13, fruit, coloration
Groups involved
Online Articles (71)