Articles
A simplified Agrobacterium-mediated transient gene expression method for functional validation of resistance genes to Neopestalotiopsis disease in strawberry (Fragaria × ananassa)
Article number
1465_27
Pages
167 – 172
Language
English
Abstract
Neopestalotiopsis spp. is a fungal pathogen that causes leaf spot and root rot diseases, significantly reducing the yield of cultivated strawberry (Fragaria × ananassa Duch). Identifying genes confer resistance to Neopestalotiopsis spp. and incorporating them into breeding programs using advanced plant breeding technologies such as CRISPR gene editing is a key strategy for improving modern strawberry cultivars.
We identified two candida genes (EDRN1 and EDRN2) associated with resistance to Neopestalotiopsis diseases using reverse genetic approaches.
To effectively validate their functions, a rapid Agrobacterium-mediated transient gene expression system was developed in strawberry plants.
This study investigates the functions of EDRN1 and EDRN2 by performing the RNAi-mediated transient assay.
Disease symptoms were reduced in both EDRN1 and/or EDRN2 knock-down plants compared to empty vector control plants.
This study highlights the importance of these genes in resistance against Neopestalotiopsis spp., providing valuable insights into the molecular mechanisms of resistance in strawberries.
This study demonstrates the method of Agrobacterium-based transient transformation and gene expression, as well as efficient cloning of target genes into RNAi vectors.
The results of this study will be valuable to the broader strawberry research community.
We identified two candida genes (EDRN1 and EDRN2) associated with resistance to Neopestalotiopsis diseases using reverse genetic approaches.
To effectively validate their functions, a rapid Agrobacterium-mediated transient gene expression system was developed in strawberry plants.
This study investigates the functions of EDRN1 and EDRN2 by performing the RNAi-mediated transient assay.
Disease symptoms were reduced in both EDRN1 and/or EDRN2 knock-down plants compared to empty vector control plants.
This study highlights the importance of these genes in resistance against Neopestalotiopsis spp., providing valuable insights into the molecular mechanisms of resistance in strawberries.
This study demonstrates the method of Agrobacterium-based transient transformation and gene expression, as well as efficient cloning of target genes into RNAi vectors.
The results of this study will be valuable to the broader strawberry research community.
Publication
Authors
S. Park, Y.J. Jang, M.B. Lee, S. Lee
Keywords
disease resistance, EDRN1, EDRN2, mutation, RNA interference
Groups involved
- Division Vine and Berry Fruits
- Working Group Strawberry Culture and Management
- Division Sustaining Horticulture in a Changing World
- Division Horticulture for Human Health
- Division Plant Genetic Resources, Breeding and Biotechnology
- Division Greenhouse and Indoor Production Horticulture
- Division Precision Horticulture and Engineering
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