Articles
Distribution of abscisic acid in strawberry fruit visualized utilizing MALDI-TOF MSI
Article number
1465_29
Pages
179 – 186
Language
English
Abstract
Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry imaging (MALDI-TOF MSI) is a powerful tool which can visualize the spatial distribution of various compounds in a tissue specimen.
While distribution of soluble carbohydrates, organic acids and anthocyanins in strawberry fruits has been examined utilizing MALDI-TOF MSI, few trails have been done on plant hormones.
It is well known that strawberry fruits contain abscisic acid (ABA), which plays an important role in fruit maturation.
Furthermore, ABA may act as an inhibitor of seed germination in strawberry fruits, as was reported for watermelon fruits.
However, since the concentration of ABA in the fruits is quite low, detecting ABA using MALDI-TOF MSI was not a viable method.
So, we tried to visualize the distribution of ABA in a strawberry fruit following Enomoto’s method, where the specimen was pretreated with Girard’s reagent T (GirT) which can assist the ionization of ABA molecules.
The results showed that the concentration of ABA was high in cortex tissue, and accumulation of ABA was clearly confirmed at the vascular bundle conjugated with achenes.
This finding may lead to understanding of the mechanisms of fruit maturation and the inhibition of seed germination in strawberry fruits.
While distribution of soluble carbohydrates, organic acids and anthocyanins in strawberry fruits has been examined utilizing MALDI-TOF MSI, few trails have been done on plant hormones.
It is well known that strawberry fruits contain abscisic acid (ABA), which plays an important role in fruit maturation.
Furthermore, ABA may act as an inhibitor of seed germination in strawberry fruits, as was reported for watermelon fruits.
However, since the concentration of ABA in the fruits is quite low, detecting ABA using MALDI-TOF MSI was not a viable method.
So, we tried to visualize the distribution of ABA in a strawberry fruit following Enomoto’s method, where the specimen was pretreated with Girard’s reagent T (GirT) which can assist the ionization of ABA molecules.
The results showed that the concentration of ABA was high in cortex tissue, and accumulation of ABA was clearly confirmed at the vascular bundle conjugated with achenes.
This finding may lead to understanding of the mechanisms of fruit maturation and the inhibition of seed germination in strawberry fruits.
Publication
Authors
T. Fujiki, H. Matsuura, Y. Jitsuyama, T. Suzuki
Keywords
abscisic acid, Fragaria × ananassa, Girard’s reagent T, mass spectrometry imaging, plant hormone
Groups involved
- Division Vine and Berry Fruits
- Working Group Strawberry Culture and Management
- Division Sustaining Horticulture in a Changing World
- Division Horticulture for Human Health
- Division Plant Genetic Resources, Breeding and Biotechnology
- Division Greenhouse and Indoor Production Horticulture
- Division Precision Horticulture and Engineering
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