Articles
A comparative study on protoplast isolation protocol from different explants of strawberry
Article number
1465_28
Pages
173 – 178
Language
English
Abstract
This study explored the efficient isolation of protoplasts from various strawberry explants, including leaf mesophyll and leaf callus.
A comparative analysis of protoplast yield and viability was conducted across these tissue types.
Leaves were harvested from in vitro-grown strawberry plants for mesophyll protoplasts.
In contrast, callus tissues were cultured on three different media: T1 (MS + 1 mg L‑1 BAP, 0.1 mg L‑1 IBA, 3% sucrose, 0.7% agar, pH 5.7), T2 (MS + 2 mg L‑1 2,4-D, 0.5 mg L‑1 kinetin, 3% sucrose, 0.7% agar, pH 5.7), and T3 (MS + 2 mg L‑1 NAA, 3% sucrose, 0.7% agar, pH 5.7). Various concentrations of mannitol, enzymes, infiltration times, and incubation periods were evaluated to optimize protoplast isolation.
The highest callus formation of all explants was observed in the T3 medium.
The results demonstrated the successful isolation of protoplasts from all explants, with potential implications for further strawberry breeding and genetic research applications.
A comparative analysis of protoplast yield and viability was conducted across these tissue types.
Leaves were harvested from in vitro-grown strawberry plants for mesophyll protoplasts.
In contrast, callus tissues were cultured on three different media: T1 (MS + 1 mg L‑1 BAP, 0.1 mg L‑1 IBA, 3% sucrose, 0.7% agar, pH 5.7), T2 (MS + 2 mg L‑1 2,4-D, 0.5 mg L‑1 kinetin, 3% sucrose, 0.7% agar, pH 5.7), and T3 (MS + 2 mg L‑1 NAA, 3% sucrose, 0.7% agar, pH 5.7). Various concentrations of mannitol, enzymes, infiltration times, and incubation periods were evaluated to optimize protoplast isolation.
The highest callus formation of all explants was observed in the T3 medium.
The results demonstrated the successful isolation of protoplasts from all explants, with potential implications for further strawberry breeding and genetic research applications.
Publication
Authors
N.E. Kafkas, S. Ates, N. Nasır, B. Mezzetti
Keywords
Fragaria × ananassa, protoplast isolation, explant, callus, protoplast viability
Groups involved
- Division Vine and Berry Fruits
- Working Group Strawberry Culture and Management
- Division Sustaining Horticulture in a Changing World
- Division Horticulture for Human Health
- Division Plant Genetic Resources, Breeding and Biotechnology
- Division Greenhouse and Indoor Production Horticulture
- Division Precision Horticulture and Engineering
Online Articles (64)