Articles
Assessment of genetic diversity obtained by regeneration from anther-culture with tomato Costate 21 cultivar
Article number
1391_38
Pages
275 – 282
Language
English
Abstract
Tomato (Solanum lycopersicon) is considered one of the most important edible crops, and represents a very important species for researchers in conventional and unconventional breeding practices and also in next generation of sequencing technology.
The biotechnological approach with in vitro androgenesis has been aroused great interest as a regenerative system for obtaining haploid and double haploid plants (pure lines) in one single generation, especially with recalcitrant or self-pollination plants, such as tomatoes.
In this study are presented some morphological and molecular characteristics of the plants obtained by in vitro androgenesis with Costate 21 cultivar.
Among the 36 regenerated tomato plants, acclimatized and grown under ex vitro conditions, 20 plants (55.6%) developed to fully maturity and were described by morphological markers (methodology recommended and developed by IPGRI). Evaluation of the phenotypic traits showed significant differences among anther-derived plants and also between anther donor cultivar (Costate 21) and androgenetic plants regarding the descriptors for: plant size and length, stem internode length, type-density and attitude of the leaf, position and shape of the style in the inflorescence, fruit characteristics (shape, size, homogeneity, flesh and core color, fruit cross-section shape). In parallel, using nine SSR markers were obtained different profiles of the DNA bands proving genetic diversity among the regenerated plants and also between them and the anthers donor cultivar.
These results with phenotypic and molecular markers bring clear evidences for the origin of the new anther-derived plants from different microspores.
The new regenerants could be considered as new genotypes valuable for tomato breeding programs.
The biotechnological approach with in vitro androgenesis has been aroused great interest as a regenerative system for obtaining haploid and double haploid plants (pure lines) in one single generation, especially with recalcitrant or self-pollination plants, such as tomatoes.
In this study are presented some morphological and molecular characteristics of the plants obtained by in vitro androgenesis with Costate 21 cultivar.
Among the 36 regenerated tomato plants, acclimatized and grown under ex vitro conditions, 20 plants (55.6%) developed to fully maturity and were described by morphological markers (methodology recommended and developed by IPGRI). Evaluation of the phenotypic traits showed significant differences among anther-derived plants and also between anther donor cultivar (Costate 21) and androgenetic plants regarding the descriptors for: plant size and length, stem internode length, type-density and attitude of the leaf, position and shape of the style in the inflorescence, fruit characteristics (shape, size, homogeneity, flesh and core color, fruit cross-section shape). In parallel, using nine SSR markers were obtained different profiles of the DNA bands proving genetic diversity among the regenerated plants and also between them and the anthers donor cultivar.
These results with phenotypic and molecular markers bring clear evidences for the origin of the new anther-derived plants from different microspores.
The new regenerants could be considered as new genotypes valuable for tomato breeding programs.
Authors
D.I. Sumedrea, A.E Alexandrescu Manolescu, C.F. Popescu, A.M. Dumitru, S.N. Cosmulescu
Keywords
androgenesis, anther-derived plants, phenotypic features, microsatellite markers, genetic variation
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